Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4664912..4665509 Replicon chromosome
Accession NZ_CP079131
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWY69_RS22725 Protein ID WP_004142563.1
Coordinates 4665192..4665509 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWY69_RS22720 Protein ID WP_004142561.1
Coordinates 4664912..4665199 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY69_RS22690 (4660992) 4660992..4661240 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWY69_RS22695 (4661258) 4661258..4661599 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWY69_RS22700 (4661630) 4661630..4662745 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWY69_RS22705 (4662925) 4662925..4663506 + 582 WP_117077156.1 TetR/AcrR family transcriptional regulator -
KWY69_RS22710 (4663506) 4663506..4663874 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWY69_RS22715 (4663994) 4663994..4664644 + 651 Protein_4451 oxygen-insensitive NAD(P)H nitroreductase -
KWY69_RS22720 (4664912) 4664912..4665199 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWY69_RS22725 (4665192) 4665192..4665509 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY69_RS22730 (4665694) 4665694..4666737 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWY69_RS22735 (4667404) 4667404..4668270 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWY69_RS22740 (4668379) 4668379..4669806 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209288 WP_004142563.1 NZ_CP079131:c4665509-4665192 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209288 NZ_CP104011:c1951227-1950946 [Pseudomonas sp. GCEP-101]
ATGAGCCTACAGTGGACGCACAAGGCAGCCGCCGACCTGGATGCCATCTACGACCATTACGTCGTGTTGATCGGCCCGGA
GAAGGCGCTGCGCGCCATCCAGGACATCGTCGAGCAGGTTCGCCCGCTGGCGAACCTGGAGCAGAGCAGCAGCGGCATGC
CCAGCGAGGTGCCCGGCGTGCGGGAATTGCCGGTGGAGCGCTGGCCGTACCAGGCGGCTTTTCGGGTGAAAGGGCGTTCT
GTACAGATTTTGCGCATTGATCGTGTGGATAACTCGGGGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209288 WP_004142561.1 NZ_CP079131:c4665199-4664912 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209288 NZ_CP104011:c1951451-1951224 [Pseudomonas sp. GCEP-101]
GTGACCACCGTTGTTTCATTTCGCGCCGATGACGCCCTCGTGGCAGCGCTGGACCAGTTGGCCCGCGCCACCCACCGGGA
CCGGCCGTATCACCTGCGCCAAGCCCTGGCGCAGTACCTGGAGCGCCAGAGCTGGCACGTGGCGGCCATCGATGAAGGGG
TGGCGGACGCCGATGCCGGCCATCTGCTGGAGCACGCCGTCATCGAAGCCAAGTGGGGCCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References