Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 633068..633665 Replicon chromosome
Accession NZ_CP079120
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC03_AI1235PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWV87_RS02970 Protein ID WP_004142563.1
Coordinates 633068..633385 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWV87_RS02975 Protein ID WP_004142561.1
Coordinates 633378..633665 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWV87_RS02955 (628771) 628771..630198 - 1428 WP_004176980.1 MFS transporter -
KWV87_RS02960 (630307) 630307..631173 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
KWV87_RS02965 (631840) 631840..632883 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
KWV87_RS02970 (633068) 633068..633385 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWV87_RS02975 (633378) 633378..633665 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWV87_RS02980 (633933) 633933..634583 - 651 Protein_586 oxygen-insensitive NAD(P)H nitroreductase -
KWV87_RS02985 (634703) 634703..635071 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KWV87_RS02990 (635071) 635071..635652 - 582 WP_117077156.1 TetR/AcrR family transcriptional regulator -
KWV87_RS02995 (635832) 635832..636947 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KWV87_RS03000 (636978) 636978..637319 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWV87_RS03005 (637337) 637337..637585 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209252 WP_004142563.1 NZ_CP079120:633068-633385 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209252 NZ_CP103974:4305345-4305602 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209252 WP_004142561.1 NZ_CP079120:633378-633665 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209252 NZ_CP103974:4305614-4306159 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References