Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4022246..4022843 Replicon chromosome
Accession NZ_CP079115
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KWK18_RS19825 Protein ID WP_004142563.1
Coordinates 4022526..4022843 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KWK18_RS19820 Protein ID WP_004142561.1
Coordinates 4022246..4022533 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWK18_RS19790 (4018341) 4018341..4018589 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KWK18_RS19795 (4018607) 4018607..4018948 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KWK18_RS19800 (4018979) 4018979..4020094 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KWK18_RS19805 (4020274) 4020274..4020858 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
KWK18_RS19810 (4020855) 4020855..4021223 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
KWK18_RS19815 (4021343) 4021343..4021981 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
KWK18_RS19820 (4022246) 4022246..4022533 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KWK18_RS19825 (4022526) 4022526..4022843 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWK18_RS19830 (4023028) 4023028..4024071 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
KWK18_RS19835 (4024741) 4024741..4025607 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KWK18_RS19840 (4025716) 4025716..4027143 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T209243 WP_004142563.1 NZ_CP079115:c4022843-4022526 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T209243 NZ_CP103974:c1972941-1972567 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT209243 WP_004142561.1 NZ_CP079115:c4022533-4022246 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT209243 NZ_CP103974:c1973398-1973030 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References