Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 335613..336199 Replicon chromosome
Accession NZ_CP079115
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KWK18_RS01570 Protein ID WP_002920800.1
Coordinates 335831..336199 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KWK18_RS01565 Protein ID WP_004174006.1
Coordinates 335613..335834 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWK18_RS01545 (331770) 331770..332696 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KWK18_RS01550 (332693) 332693..333970 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KWK18_RS01555 (333967) 333967..334734 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KWK18_RS01560 (334736) 334736..335449 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KWK18_RS01565 (335613) 335613..335834 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KWK18_RS01570 (335831) 335831..336199 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KWK18_RS01575 (336472) 336472..337788 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KWK18_RS01580 (337895) 337895..338782 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KWK18_RS01585 (338779) 338779..339624 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KWK18_RS01590 (339626) 339626..340696 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320473..341433 20960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T209232 WP_002920800.1 NZ_CP079115:335831-336199 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T209232 NZ_CP103972:c4870265-4870158 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT209232 WP_004174006.1 NZ_CP079115:335613-335834 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT209232 NZ_CP103972:4870312-4870378 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References