Detailed information of TA system TA209221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2555575..2556155
• Replicon: chromosome
• Accession: NZ_CP079095
• Organism: Methylococcus sp. Mc7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KW115_RS12555
• Protein ID: WP_218806058.1
• Coordinates: 2555877..2556155 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KW115_RS12550
• Protein ID: WP_218806057.1
• Coordinates: 2555575..2555877 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW115_RS12520 (KW115_12520)	2550891..2553245	-	2355	WP_218806051.1	DUF927 domain-containing protein	-
KW115_RS12525 (KW115_12525)	2553505..2553759	-	255	WP_218806052.1	hypothetical protein	-
KW115_RS12530 (KW115_12530)	2553752..2554039	-	288	WP_218806053.1	hypothetical protein	-
KW115_RS12535 (KW115_12535)	2554350..2554619	+	270	WP_218806054.1	DUF1640 domain-containing protein	-
KW115_RS12545 (KW115_12545)	2554962..2555474	-	513	WP_218806056.1	hypothetical protein	-
KW115_RS12550 (KW115_12550)	2555575..2555877	-	303	WP_218806057.1	HigA family addiction module antitoxin	Antitoxin
KW115_RS12555 (KW115_12555)	2555877..2556155	-	279	WP_218806058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW115_RS12560 (KW115_12560)	2556195..2557313	-	1119	WP_218806059.1	tyrosine-type recombinase/integrase	-
KW115_RS12570 (KW115_12570)	2558261..2558650	+	390	WP_218806060.1	exosortase system-associated protein, TIGR04073 family	-
KW115_RS12575 (KW115_12575)	2558927..2560849	-	1923	WP_218806061.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-88)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10546.73 Da
• Isoelectric Point: 7.5217

>T209221 WP_218806058.1 NZ_CP079095:c2556155-2555877 [Methylococcus sp. Mc7]
MIQSFKHKGLSRYFAEDDHRGINPKQAARIRRLLDRLDAAAQAEDMNLPGYGFHGLAHNRAGEYAVTVSGNWRITFRFES
GHAFDVNLEDYH

Nucleotide

• Length: 279 bp

>T209221 NZ_CP103972:c1781530-1781123 [Escherichia coli]
GTGGTTCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11324.11 Da
• Isoelectric Point: 10.0757

>AT209221 WP_218806057.1 NZ_CP079095:c2555877-2555575 [Methylococcus sp. Mc7]
MKSLRHDRRRPTHPGEVLREDVLPALGITQTELAKRLMVSRVTVSEILLEKRALSPDMAVRISRLTSTTPESWLAMQQAV
DLWEVNQHPERYAAISPVAA

Nucleotide

• Length: 303 bp

>AT209221 NZ_CP103972:c1781777-1781511 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTTCTGTGGCAATCTGA