Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2281649..2282259 Replicon chromosome
Accession NZ_CP078509
Organism Stutzerimonas stutzeri strain S116

Toxin (Protein)


Gene name higB Uniprot ID A0A482U652
Locus tag KVH38_RS10590 Protein ID WP_017244792.1
Coordinates 2281945..2282259 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID I4CQU1
Locus tag KVH38_RS10585 Protein ID WP_003302338.1
Coordinates 2281649..2281939 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVH38_RS10555 (KVH38_10555) 2276821..2277030 + 210 WP_218650712.1 cold-shock protein -
KVH38_RS10560 (KVH38_10560) 2277135..2278229 - 1095 WP_020307256.1 iron-sulfur cluster carrier protein ApbC -
KVH38_RS10565 (KVH38_10565) 2278400..2280439 + 2040 WP_125919738.1 methionine--tRNA ligase -
KVH38_RS10570 (KVH38_10570) 2280497..2280703 + 207 WP_125919737.1 cysteine-rich CWC family protein -
KVH38_RS10575 (KVH38_10575) 2280703..2281395 + 693 WP_125856319.1 16S rRNA pseudouridine(516) synthase -
KVH38_RS10585 (KVH38_10585) 2281649..2281939 - 291 WP_003302338.1 helix-turn-helix domain-containing protein Antitoxin
KVH38_RS10590 (KVH38_10590) 2281945..2282259 - 315 WP_017244792.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KVH38_RS10595 (KVH38_10595) 2282304..2283311 - 1008 WP_239329965.1 tyrosine-type recombinase/integrase -
KVH38_RS10600 (KVH38_10600) 2283311..2284588 - 1278 WP_125865222.1 hypothetical protein -
KVH38_RS10605 (KVH38_10605) 2284736..2285920 - 1185 WP_125865221.1 zonular occludens toxin domain-containing protein -
KVH38_RS10610 (KVH38_10610) 2285933..2286259 - 327 WP_125856689.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2281522..2294844 13322


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11953.71 Da        Isoelectric Point: 9.8035

>T209197 WP_017244792.1 NZ_CP078509:c2282259-2281945 [Stutzerimonas stutzeri]
MIFIETPIFTKRLRDLLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASSGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Download         Length: 315 bp

>T209197 NZ_CP103901:c739877-739458 [Corynebacterium diphtheriae bv. gravis]
ATGGTGAAAAAGTTGGTCTACTTATCCTGCACCTTAAATCAGAAGTGCGGGCAGCGAACAGGTCCCCACAGAGAACCTGA
ACCTTTTCTTGGATTCTCCGTGAGGGATCGGAGCGGGCTAGAGGGCGCGATCGCTTCAGTATTTCAATCGTCGTTTGACG
TCTTGCTGTACCCGACCATATACGACCAAGCGGCCGCGCTCCTGTTCAAGCTGGCAAACGGGCATTACTTCCAAGACGGG
AACAAGCGAACAGCATTCCACCTGTGCTTAGCCTACCTACGGGCCAATAACGTTCACGTCAGTCCCCCTCGGTCTGATGA
CGTCATCACTTTACTTCAGACCACGGTGAGTCGCTCAGGAAGCCAGGAAAACGGGATAGCGTTCGTTAGACAAAAGCTTT
TGGACTGGACCGTAGACTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10925.51 Da        Isoelectric Point: 8.8068

>AT209197 WP_003302338.1 NZ_CP078509:c2281939-2281649 [Stutzerimonas stutzeri]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Download         Length: 291 bp

>AT209197 NZ_CP103901:c740488-739922 [Corynebacterium diphtheriae bv. gravis]
ATGATCAGCATCCTCCATGTTGCAGCGCGTGTACTTGAGCTGCGCCCTTCCTGCGACAAGATGAAGCTGTACAAGCTGTG
TTACTTCTCCCAGGGTTGGCACTTGGCGTGGACAGGTCGTCCGCTGTTCAACGAAGAGCTACAGGCGTGGAAGTACGGGG
CCGTATCTCCTACCTTGCGTCAGGCTAGCGGGCTTCGTGCCGATGATGACCGTCTAGTGACCCAAATTTGGAGCGGGGAC
AGTTCCCAACTCATCGATTACGAGCGGTCAGTCGTGGACACTGTTGTTTCGTTCTATGGCGATCTTGAGTCCTTTCACCT
GTCCGATTTGAGCCACGGCTTCGCCTGGTCCACCGTACGAGGCAACCTCCCTCCAGACGCCTCGTGCAGCGATGTGATCC
CTCACTCTCTGATCCGTCGTGAGTTTGTCGAGAATGCTTGGGGCGAAGCTCCTACGCCAAACGCTCCTGAACGCCTACCT
TCCATGGCACTTGATGCTCTCGAGCAGGCAGCCGATGACGTTGCTGCCGAAAATGCAGAGACGCTTCGACTTCTCGCTTT
CATCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A482U652

Antitoxin

Source ID Structure
AlphaFold DB I4CQU1

References