Detailed information of TA system TA209140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5030444..5031078
• Replicon: chromosome
• Accession: NZ_CP078146
• Organism: Klebsiella variicola strain JNQH579

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KW408_RS24390
• Protein ID: WP_218408226.1
• Coordinates: 5030743..5031078 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: KW408_RS24385
• Protein ID: WP_008806992.1
• Coordinates: 5030444..5030743 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW408_RS24365 (KW408_24365)	5026650..5027408	-	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-
KW408_RS24370 (KW408_24370)	5027458..5028288	-	831	WP_008806994.1	rhomboid family intramembrane serine protease GlpG	-
KW408_RS24375 (KW408_24375)	5028339..5028668	-	330	WP_004202138.1	thiosulfate sulfurtransferase GlpE	-
KW408_RS24380 (KW408_24380)	5028873..5030381	+	1509	WP_032740278.1	glycerol-3-phosphate dehydrogenase	-
KW408_RS24385 (KW408_24385)	5030444..5030743	-	300	WP_008806992.1	XRE family transcriptional regulator	Antitoxin
KW408_RS24390 (KW408_24390)	5030743..5031078	-	336	WP_218408226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW408_RS24395 (KW408_24395)	5031971..5031972	-	2	WP_218408074.1	IS3 family transposase	-
KW408_RS24400 (KW408_24400)	5032640..5035087	-	2448	WP_008806990.1	glycogen phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12715.69 Da
• Isoelectric Point: 9.0053

>T209140 WP_218408226.1 NZ_CP078146:c5031078-5030743 [Klebsiella variicola]
VSGNIGANPFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAIVLCA
GNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 336 bp

>T209140 NZ_CP103863:1043335-1043478 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT209140 WP_008806992.1 NZ_CP078146:c5030743-5030444 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT209140 NZ_CP103863:c1043595-1043411 [Enterococcus faecalis]
TGCTACAATAGAGATGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZT5