Detailed information of TA system TA209123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3634780..3635378
• Replicon: chromosome
• Accession: NZ_CP078145
• Organism: Nocardia iowensis strain NRRL 5646

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KV110_RS16775
• Protein ID: WP_218477149.1
• Coordinates: 3635100..3635378 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KV110_RS16770
• Protein ID: WP_246634590.1
• Coordinates: 3634780..3635088 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KV110_RS16735 (KV110_16735)	3630115..3630318	+	204	WP_218477137.1	ferredoxin	-
KV110_RS16740 (KV110_16740)	3630329..3630559	+	231	WP_218477138.1	biotin/lipoyl-containing protein	-
KV110_RS16745 (KV110_16745)	3630615..3631379	+	765	WP_218477139.1	SDR family oxidoreductase	-
KV110_RS16750 (KV110_16750)	3631437..3632204	+	768	WP_218477140.1	ThuA domain-containing protein	-
KV110_RS16755 (KV110_16755)	3632736..3633206	+	471	WP_218477142.1	hypothetical protein	-
KV110_RS16760 (KV110_16760)	3633749..3633910	-	162	WP_218477145.1	hypothetical protein	-
KV110_RS16765 (KV110_16765)	3634070..3634243	+	174	WP_218477147.1	site-specific integrase	-
KV110_RS16770 (KV110_16770)	3634780..3635088	-	309	WP_246634590.1	HigA family addiction module antitoxin	Antitoxin
KV110_RS16775 (KV110_16775)	3635100..3635378	-	279	WP_218477149.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KV110_RS41795	3635958..3636251	+	294	WP_281427769.1	DUF3578 domain-containing protein	-
KV110_RS16785 (KV110_16785)	3636308..3637231	+	924	WP_218477152.1	DUF3883 domain-containing protein	-
KV110_RS16795 (KV110_16795)	3638534..3639919	-	1386	WP_218477154.1	SEC-C domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10523.00 Da
• Isoelectric Point: 9.1204

>T209123 WP_218477149.1 NZ_CP078145:c3635378-3635100 [Nocardia iowensis]
VIQSFKDGEAQKLWTERSAPKLGQLQTVALRKLTMLDAAAQLNDLRAPPANRLEKLRGDREGQHSIRINDKWRICFAWEA
AGPRDVEIVDYH

Nucleotide

• Length: 279 bp

>T209123 NZ_CP103856:c1272553-1271807 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGATGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11461.32 Da
• Isoelectric Point: 10.6679

>AT209123 WP_246634590.1 NZ_CP078145:c3635088-3634780 [Nocardia iowensis]
MAPTTPLPPTSPGEILLEEFMKPLGISQNALARALGVSPRRINQIVNGKRAITTGTALRLARYFSTTPEFWINLQTRYDL
VLERENLGDKLSRIVPRERVNC

Nucleotide

• Length: 309 bp

>AT209123 NZ_CP103856:c1271806-1271636 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGCTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA