Detailed information of TA system TA209092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3996761..3997363
• Replicon: chromosome
• Accession: NZ_CP078137
• Organism: Escherichia coli strain Sx181-0-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KW063_RS20005
• Protein ID: WP_000897305.1
• Coordinates: 3997052..3997363 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KW063_RS20000
• Protein ID: WP_000356397.1
• Coordinates: 3996761..3997051 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW063_RS19975 (3992706)	3992706..3993608	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KW063_RS19980 (3993605)	3993605..3994240	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KW063_RS19985 (3994237)	3994237..3995166	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KW063_RS19990 (3995496)	3995496..3995738	-	243	WP_001087409.1	protein YiiF	-
KW063_RS19995 (3995957)	3995957..3996175	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KW063_RS20000 (3996761)	3996761..3997051	-	291	WP_000356397.1	NadS family protein	Antitoxin
KW063_RS20005 (3997052)	3997052..3997363	-	312	WP_000897305.1	hypothetical protein	Toxin
KW063_RS20010 (3997592)	3997592..3998500	+	909	WP_001162704.1	alpha/beta hydrolase	-
KW063_RS20015 (3998564)	3998564..3999505	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KW063_RS20020 (3999550)	3999550..3999987	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KW063_RS20025 (3999984)	3999984..4000856	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KW063_RS20030 (4000850)	4000850..4001449	-	600	WP_001295269.1	glucose-1-phosphatase	-
KW063_RS20035 (4001548)	4001548..4002333	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T209092 WP_000897305.1 NZ_CP078137:c3997363-3997052 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T209092 NZ_CP103832:2295296-2295667 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT209092 WP_000356397.1 NZ_CP078137:c3997051-3996761 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT209092 NZ_CP103832:2295648-2295938 [Actinobacillus equuli subsp. haemolyticus]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7