Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3150040..3150581 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag KW063_RS16025 Protein ID WP_000615982.1
Coordinates 3150303..3150581 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KW063_RS16020 Protein ID WP_000729704.1
Coordinates 3150040..3150300 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS15995 (3145823) 3145823..3146608 - 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -
KW063_RS16000 (3146580) 3146580..3148292 + 1713 Protein_2990 flagellar biosynthesis protein FlhA -
KW063_RS16005 (3148397) 3148397..3148675 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
KW063_RS16010 (3148668) 3148668..3149024 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KW063_RS16015 (3149081) 3149081..3149854 - 774 WP_000543895.1 C40 family peptidase -
KW063_RS16020 (3150040) 3150040..3150300 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KW063_RS16025 (3150303) 3150303..3150581 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KW063_RS16030 (3150737) 3150737..3151477 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KW063_RS16035 (3151448) 3151448..3152215 - 768 WP_000333380.1 class II glutamine amidotransferase -
KW063_RS16040 (3152421) 3152421..3152999 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T209090 WP_000615982.1 NZ_CP078137:3150303-3150581 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T209090 NZ_CP103831:2322940-2323311 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACAATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTTTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT209090 WP_000729704.1 NZ_CP078137:3150040-3150300 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT209090 NZ_CP103831:2323292-2323582 [Actinobacillus equuli subsp. haemolyticus]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTTCG
TGTTAAATTGATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References