Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 49559..50358 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name yhaV Uniprot ID A0A6H2GMC5
Locus tag KW063_RS00985 Protein ID WP_000347279.1
Coordinates 49559..50023 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KW063_RS00990 Protein ID WP_001307405.1
Coordinates 50023..50358 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS00955 (44560) 44560..44994 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KW063_RS00960 (45012) 45012..45890 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KW063_RS00965 (45880) 45880..46659 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KW063_RS00970 (46670) 46670..47143 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KW063_RS00975 (47166) 47166..48446 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KW063_RS00980 (48695) 48695..49504 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KW063_RS00985 (49559) 49559..50023 - 465 WP_000347279.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KW063_RS00990 (50023) 50023..50358 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KW063_RS00995 (50507) 50507..52078 - 1572 WP_001273741.1 galactarate dehydratase -
KW063_RS01000 (52453) 52453..53787 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KW063_RS01005 (53803) 53803..54573 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17869.24 Da        Isoelectric Point: 9.4947

>T209072 WP_000347279.1 NZ_CP078137:c50023-49559 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Download         Length: 465 bp

>T209072 NZ_CP103825:1960558-1960920 [Actinobacillus equuli subsp. haemolyticus]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGAAATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT209072 WP_001307405.1 NZ_CP078137:c50358-50023 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT209072 NZ_CP103825:1960904-1961212 [Actinobacillus equuli subsp. haemolyticus]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGCCCGTCTGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACTAAACCGAATCCA
CAAGCTACTTTGTTATTACGAATGGTGGACAAATCTCCGCAATTATTAGAACAAATGGCACAGCTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GMC5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References