Detailed information of TA system TA209066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 106218..106945
• Replicon: plasmid pKP169-P1
• Accession: NZ_CP078124
• Organism: Klebsiella pneumoniae strain KPC-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KU889_RS29105
• Protein ID: WP_011251285.1
• Coordinates: 106218..106529 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU889_RS29110
• Protein ID: WP_011251286.1
• Coordinates: 106526..106945 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU889_RS29065	101229..101723	+	495	WP_011251274.1	hypothetical protein	-
KU889_RS29070	101729..102169	+	441	WP_011251275.1	hypothetical protein	-
KU889_RS29075	102594..103550	-	957	WP_011251280.1	DsbA family protein	-
KU889_RS29080	103610..103951	-	342	WP_011251281.1	hypothetical protein	-
KU889_RS29085	103965..104276	-	312	WP_011251282.1	hypothetical protein	-
KU889_RS29090	104293..104742	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
KU889_RS29100	105576..106013	+	438	Protein_124	DDE-type integrase/transposase/recombinase	-
KU889_RS29105	106218..106529	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KU889_RS29110	106526..106945	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KU889_RS29115	107092..108060	+	969	WP_074428168.1	IS5 family transposase	-
KU889_RS29120	108132..108497	-	366	WP_048333448.1	hypothetical protein	-
KU889_RS29125	108511..109299	-	789	WP_040217257.1	hypothetical protein	-
KU889_RS29130	109320..109940	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
KU889_RS29135	110359..110994	+	636	WP_223171879.1	hypothetical protein	-
KU889_RS29140	111307..111777	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..221577	221577
	-	inside	IScluster/Tn	-	-	99309..123616	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T209066 WP_011251285.1 NZ_CP078124:106218-106529 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T209066 NZ_CP103822:2139533-2139874 [Actinobacillus equuli subsp. haemolyticus]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGATAAATTTCGTTATGAAAATTTAACAGATGATGATTATCGTGAATT
TCAAGAATATTTGATGAATAATCCCGAAGCTGGAGATATTGTTCAAGGCGTAAACGGTTTACGAAAAATTCGTATATCAA
CCCAAGCAAAGGGGAAGAAAGGTGGTGCCAGAGTGATTTACTACTATTATGTTAGAGGATCACAAATTTGGTTATTTACA
GGGTATAACAAATCAAGAAAAATTGATTTGAATTCAATGGAAAGAAAAGCATTTTCTAAAGTATTAGAACATTTAAAAAA
TATTGCGGATAGGGAGCTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT209066 WP_011251286.1 NZ_CP078124:106526-106945 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT209066 NZ_CP103822:2139875-2140183 [Actinobacillus equuli subsp. haemolyticus]
ATGGGACGTTTATTTGATGATCTGATGGAAGGGGCGGAAGCCTTAGAACAATACCTTCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCGATAGAACTTAGCCCGTCCGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTACATACCTCCGTGCGGACTTATCAAGGGTGGGAACAAGGTAAAACCAAACCGAATCCA
CAAGCAGTGCTGTTGCTTCGTATGGTGGATAAATCGCCTCAGTTATTAGAACAAATGGCACAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1