Detailed information of TA system TA209057

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72698..73124
• Replicon: plasmid pKP169-P2
• Accession: NZ_CP078123
• Organism: Klebsiella pneumoniae strain KPC-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KU889_RS27925
• Protein ID: WP_001372321.1
• Coordinates: 72698..72823 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72900..73124 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU889_RS27890 (67753)	67753..67980	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
KU889_RS27895 (68074)	68074..68760	-	687	WP_015059009.1	PAS domain-containing protein	-
KU889_RS27900 (68951)	68951..69334	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KU889_RS27905 (69611)	69611..70258	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
KU889_RS27910 (70555)	70555..71376	-	822	WP_001234445.1	DUF932 domain-containing protein	-
KU889_RS27915 (71487)	71487..71783	-	297	WP_001272251.1	hypothetical protein	-
KU889_RS27920 (72083)	72083..72379	+	297	Protein_96	hypothetical protein	-
KU889_RS27925 (72698)	72698..72823	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KU889_RS30865 (72765)	72765..72914	-	150	Protein_98	plasmid maintenance protein Mok	-
- (72900)	72900..73124	-	225	NuclAT_0	-	Antitoxin
- (72900)	72900..73124	-	225	NuclAT_0	-	Antitoxin
- (72900)	72900..73124	-	225	NuclAT_0	-	Antitoxin
- (72900)	72900..73124	-	225	NuclAT_0	-	Antitoxin
KU889_RS27930 (73136)	73136..73855	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
KU889_RS27935 (73852)	73852..74286	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KU889_RS27940 (74355)	74355..76378	-	2024	Protein_101	ParB/RepB/Spo0J family partition protein	-
KU889_RS27945 (76439)	76439..76672	-	234	WP_000006003.1	DUF905 family protein	-
KU889_RS27950 (76730)	76730..77257	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
KU889_RS30870 (77559)	77559..78020	+	462	Protein_104	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3	-	1..157394	157394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T209057 WP_001372321.1 NZ_CP078123:c72823-72698 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T209057 NZ_CP103818:1990273-1990635 [Actinobacillus equuli subsp. haemolyticus]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT209057 NZ_CP078123:c73124-72900 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin