Detailed information of TA system TA209025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 801782..802392
• Replicon: chromosome
• Accession: NZ_CP078120
• Organism: Halomonas sp. 18071143

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KVY02_RS03895
• Protein ID: WP_100731017.1
• Coordinates: 801782..802063 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: G6YV52
• Locus tag: KVY02_RS03900
• Protein ID: WP_008174257.1
• Coordinates: 802078..802392 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVY02_RS03865	797153..798027	+	875	Protein_745	transposase	-
KVY02_RS03870	798342..799199	+	858	WP_213262753.1	site-specific integrase	-
KVY02_RS03875	799192..800343	+	1152	WP_218314437.1	IS91 family transposase	-
KVY02_RS03880	800680..800979	+	300	WP_218314438.1	hypothetical protein	-
KVY02_RS03885	801108..801365	+	258	WP_081136158.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KVY02_RS03890	801353..801655	+	303	WP_218314439.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KVY02_RS03895	801782..802063	+	282	WP_100731017.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KVY02_RS03900	802078..802392	+	315	WP_008174257.1	HigA family addiction module antitoxin	Antitoxin
KVY02_RS03905	802492..802719	+	228	WP_218314440.1	hypothetical protein	-
KVY02_RS03910	802841..802996	+	156	WP_218314442.1	hypothetical protein	-
KVY02_RS03920	803394..803492	+	99	Protein_755	IS5/IS1182 family transposase	-
KVY02_RS03925	803621..804271	+	651	WP_081136178.1	peroxiredoxin	-
KVY02_RS03930	804311..805579	-	1269	WP_218314444.1	PAS domain-containing methyl-accepting chemotaxis protein	-
KVY02_RS03935	805711..806724	-	1014	WP_100731020.1	isocitrate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	780904..803543	22639
	-	flank	IS/Tn	-	-	796739..797107	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10663.11 Da
• Isoelectric Point: 8.4897

>T209025 WP_100731017.1 NZ_CP078120:801782-802063 [Halomonas sp. 18071143]
MIKSFADKRTQELYSKGKSKKFPADVAPRAARKLEYVDLALQLEDLKVPPGNRLHPLSGNRQGQHAISINDQWRICFRFE
GGDAYEVEVCDYH

Nucleotide

• Length: 282 bp

>T209025 NZ_CP103807:c1424460-1424062 [Actinobacillus genomosp. 2]
ATGCTGACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCCACTCGTCTTTGCGTGAGTAGTATTACAGCGGCTGAGTTATATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTCATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGTAATATCAAAGCACATTTATCTAAGCAAGGTGAGATCATCGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTACGATTAGCTAATTGGGTATAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11644.33 Da
• Isoelectric Point: 7.2052

>AT209025 WP_008174257.1 NZ_CP078120:802078-802392 [Halomonas sp. 18071143]
MAILNTAGMQRKPTHPGEMLREDFLPDYGLTVSGLAESLGVSRQSVNELLRERRAVSPEMALRLARLFGNSPEFWLNAQR
SVDLWTAAQSVKEEVDRIKPLHAA

Nucleotide

• Length: 315 bp

>AT209025 NZ_CP103807:c1424690-1424460 [Actinobacillus genomosp. 2]
ATGGAAGCATCAGTATTTATGACAAATCGCAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTATAAGAGTAGTCGGTAATGAGCGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTTATGACAGAGCGTGAAAGTAGCACTCAAGCCGAACGTGAGGAACTCTAA

Structures

Antitoxin

• AlphaFold DB: G6YV52