Detailed information of TA system TA209008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4266865..4267466
• Replicon: chromosome
• Accession: NZ_CP078110
• Organism: Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A855WMY7
• Locus tag: BTK77_RS20310
• Protein ID: WP_054623952.1
• Coordinates: 4266865..4267176 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BTK77_RS20315
• Protein ID: WP_007871962.1
• Coordinates: 4267176..4267466 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTK77_RS20295 (BTK77_020290)	4262223..4264190	-	1968	WP_080321226.1	glycoside hydrolase family 127 protein	-
BTK77_RS20300 (BTK77_020295)	4264202..4265605	-	1404	Protein_3963	MFS transporter	-
BTK77_RS20305 (BTK77_020300)	4265866..4266690	+	825	WP_080321227.1	AraC family transcriptional regulator	-
BTK77_RS20310 (BTK77_020305)	4266865..4267176	+	312	WP_054623952.1	hypothetical protein	Toxin
BTK77_RS20315 (BTK77_020310)	4267176..4267466	+	291	WP_007871962.1	NadS family protein	Antitoxin
BTK77_RS20320 (BTK77_020315)	4267540..4268469	-	930	WP_007871963.1	formate dehydrogenase accessory protein FdhE	-
BTK77_RS20325 (BTK77_020320)	4268466..4269101	-	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
BTK77_RS20330 (BTK77_020325)	4269098..4270000	-	903	WP_004385164.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12155.11 Da
• Isoelectric Point: 9.7588

>T209008 WP_054623952.1 NZ_CP078110:4266865-4267176 [Cronobacter sakazakii]
MLFIETEIFTRAVTALLSDDDYQRLQWFLALRPDYGDVIPDTGGLRKVRWLSGGRGKRGGVRIIYFYRPVDDEIRLLLIY
RKGIKDDLSPHEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T209008 NZ_CP103791:204608-205093 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10659.23 Da
• Isoelectric Point: 10.7279

>AT209008 WP_007871962.1 NZ_CP078110:4267176-4267466 [Cronobacter sakazakii]
MDKALFEQLTESLRQMNDISEGRREPSRVFVIDALKIKAIREASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVLSALAL

Nucleotide

• Length: 291 bp

>AT209008 NZ_CP103791:204333-204620 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCAGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A855WMY7