Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2549208..2549855 Replicon chromosome
Accession NZ_CP078110
Organism Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BTK77_RS12035 Protein ID WP_080320329.1
Coordinates 2549508..2549855 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V5TYW5
Locus tag BTK77_RS12030 Protein ID WP_007776551.1
Coordinates 2549208..2549507 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTK77_RS12000 (BTK77_012000) 2544618..2545184 + 567 WP_063264316.1 DedA family protein -
BTK77_RS12005 (BTK77_012005) 2545181..2545954 - 774 WP_032988288.1 SDR family oxidoreductase -
BTK77_RS12010 (BTK77_012010) 2546127..2546210 + 84 WP_000691708.1 small membrane protein YohP -
BTK77_RS12015 (BTK77_012015) 2546247..2547206 - 960 WP_080320330.1 MBL fold metallo-hydrolase -
BTK77_RS12020 (BTK77_012020) 2547279..2548202 + 924 WP_054624201.1 LysR family transcriptional regulator -
BTK77_RS12025 (BTK77_012025) 2548205..2549137 - 933 WP_032988286.1 tRNA dihydrouridine(16) synthase DusC -
BTK77_RS12030 (BTK77_012030) 2549208..2549507 - 300 WP_007776551.1 XRE family transcriptional regulator Antitoxin
BTK77_RS12035 (BTK77_012035) 2549508..2549855 - 348 WP_080320329.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BTK77_RS12040 (BTK77_012040) 2550108..2550509 + 402 WP_007869367.1 CidA/LrgA family protein -
BTK77_RS12045 (BTK77_012045) 2550506..2551201 + 696 WP_007869369.1 CidB/LrgB family autolysis modulator -
BTK77_RS12050 (BTK77_012050) 2551331..2552215 + 885 WP_080320328.1 cytidine deaminase -
BTK77_RS12055 (BTK77_012055) 2552340..2553053 + 714 WP_004387784.1 outer membrane permeability protein SanA -
BTK77_RS12060 (BTK77_012060) 2553318..2554328 - 1011 WP_004387466.1 galactose/methyl galactoside ABC transporter permease MglC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-83)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13300.55 Da        Isoelectric Point: 10.2065

>T209002 WP_080320329.1 NZ_CP078110:c2549855-2549508 [Cronobacter sakazakii]
MWTIYLTHEFECWLAGQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKALRVQHAGRPIRAFYAFDPHRYAI
VLCAAQKKGDEKRFYRVMLRVADKLFSHYLNHREV

Download         Length: 348 bp

>T209002 NZ_CP103783:c2219419-2219243 [Bacillus subtilis subsp. subtilis str. 168]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11035.67 Da        Isoelectric Point: 5.1667

>AT209002 WP_007776551.1 NZ_CP078110:c2549507-2549208 [Cronobacter sakazakii]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Download         Length: 300 bp

>AT209002 NZ_CP103783:2219202-2219302 [Bacillus subtilis subsp. subtilis str. 168]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UA72

References