Detailed information of TA system TA208989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 36571..37087
• Replicon: plasmid unnamed
• Accession: NZ_CP078098
• Organism: Lacticaseibacillus paracasei subsp. paracasei strain GR0548

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: J5Y16_RS14245
• Protein ID: WP_218210374.1
• Coordinates: 36571..36852 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: J5Y16_RS14250
• Protein ID: WP_016363783.1
• Coordinates: 36845..37087 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5Y16_RS14225 (J5Y16_14225)	31793..32470	-	678	WP_218210371.1	type 1 glutamine amidotransferase	-
J5Y16_RS14230 (J5Y16_14230)	32648..33016	-	369	WP_218210372.1	YxeA family protein	-
J5Y16_RS14235 (J5Y16_14235)	33554..34750	+	1197	WP_218210373.1	MFS transporter	-
J5Y16_RS14240 (J5Y16_14240)	34890..36130	-	1241	Protein_38	ISL3 family transposase	-
J5Y16_RS14245 (J5Y16_14245)	36571..36852	-	282	WP_218210374.1	type II toxin-antitoxin system YafQ family toxin	Toxin
J5Y16_RS14250 (J5Y16_14250)	36845..37087	-	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J5Y16_RS14255 (J5Y16_14255)	37322..37807	+	486	WP_202664753.1	hypothetical protein	-
J5Y16_RS14260 (J5Y16_14260)	38601..39083	+	483	WP_003577506.1	PTS sugar transporter subunit IIB	-
J5Y16_RS14265 (J5Y16_14265)	39096..40013	+	918	WP_218210328.1	PTS sugar transporter subunit IIC	-
J5Y16_RS14270 (J5Y16_14270)	40000..40821	+	822	WP_109990538.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..75538	75538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11397.03 Da
• Isoelectric Point: 9.1895

>T208989 WP_218210374.1 NZ_CP078098:c36852-36571 [Lacticaseibacillus paracasei subsp. paracasei]
MNKFIYKPAFERQFKKHYKTILKGGLYKKEDFEAVYHKLLRDEPLDPHYNDHPLVNRKPERDLHIRPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T208989 NZ_CP103771:c3793941-3793528 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT208989 WP_016363783.1 NZ_CP078098:c37087-36845 [Lacticaseibacillus paracasei subsp. paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT208989 NZ_CP103771:c3794188-3793922 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA