Detailed information of TA system TA208986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 70463..71027
• Replicon: chromosome
• Accession: NZ_CP078097
• Organism: Lacticaseibacillus paracasei subsp. paracasei strain GR0548

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: J5Y16_RS00315
• Protein ID: WP_218209856.1
• Coordinates: 70463..70765 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: J5Y16_RS00320
• Protein ID: WP_218209857.1
• Coordinates: 70758..71027 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5Y16_RS00295 (J5Y16_00295)	65776..66333	+	558	WP_003565104.1	helix-turn-helix transcriptional regulator	-
J5Y16_RS00300 (J5Y16_00300)	66758..68935	-	2178	WP_218209855.1	Tex family protein	-
J5Y16_RS00305 (J5Y16_00305)	69171..69863	-	693	WP_003570055.1	hypothetical protein	-
J5Y16_RS00310 (J5Y16_00310)	69981..70124	-	144	WP_162259784.1	hypothetical protein	-
J5Y16_RS00315 (J5Y16_00315)	70463..70765	-	303	WP_218209856.1	type II toxin-antitoxin system YafQ family toxin	Toxin
J5Y16_RS00320 (J5Y16_00320)	70758..71027	-	270	WP_218209857.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J5Y16_RS00325 (J5Y16_00325)	71401..72054	-	654	WP_016374847.1	N-acetyltransferase	-
J5Y16_RS00330 (J5Y16_00330)	72099..72727	-	629	Protein_64	DUF2399 domain-containing protein	-
J5Y16_RS00335 (J5Y16_00335)	72790..73116	-	327	WP_003584272.1	YrdB family protein	-
J5Y16_RS00340 (J5Y16_00340)	73109..73513	-	405	WP_003570045.1	MerR family transcriptional regulator	-
J5Y16_RS00345 (J5Y16_00345)	73711..74937	+	1227	WP_003570043.1	hypothetical protein	-
J5Y16_RS00350 (J5Y16_00350)	75134..76021	+	888	WP_003570041.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-98)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11497.29 Da
• Isoelectric Point: 10.1555

>T208986 WP_218209856.1 NZ_CP078097:c70765-70463 [Lacticaseibacillus paracasei subsp. paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKRAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T208986 NZ_CP103771:2590328-2590639 [Salmonella enterica]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAAAGATTTCTCTTCTCCGGCAGAGCTACGAAAGGTCATACCGAGTCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTACTACAGGGAGAATAAAGAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10000.72 Da
• Isoelectric Point: 7.2026

>AT208986 WP_218209857.1 NZ_CP078097:c71027-70758 [Lacticaseibacillus paracasei subsp. paracasei]
MAATKKETRLNMRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT208986 NZ_CP103771:2590636-2591055 [Salmonella enterica]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTGGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGCGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA