Detailed information of TA system TA208975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1280698..1281308
• Replicon: chromosome
• Accession: NZ_CP078087
• Organism: Stutzerimonas stutzeri strain YWX-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N2IK93
• Locus tag: KUD99_RS06035
• Protein ID: WP_010799516.1
• Coordinates: 1280698..1281012 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUD99_RS06040
• Protein ID: WP_010799517.1
• Coordinates: 1281018..1281308 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUD99_RS06015	1276695..1277021	+	327	WP_181090431.1	DUF2523 domain-containing protein	-
KUD99_RS06020	1277034..1278221	+	1188	WP_218422356.1	zonular occludens toxin domain-containing protein	-
KUD99_RS06025	1278369..1279646	+	1278	WP_218422571.1	hypothetical protein	-
KUD99_RS06030	1279646..1280653	+	1008	WP_256332507.1	tyrosine-type recombinase/integrase	-
KUD99_RS06035	1280698..1281012	+	315	WP_010799516.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUD99_RS06040	1281018..1281308	+	291	WP_010799517.1	helix-turn-helix domain-containing protein	Antitoxin
KUD99_RS06050	1281562..1282254	-	693	WP_108241596.1	16S rRNA pseudouridine(516) synthase	-
KUD99_RS06055	1282254..1282460	-	207	WP_218422358.1	cysteine-rich CWC family protein	-
KUD99_RS06060	1282518..1284557	-	2040	WP_218422359.1	methionine--tRNA ligase	-
KUD99_RS06065	1284728..1285822	+	1095	WP_011912443.1	iron-sulfur cluster carrier protein ApbC	-
KUD99_RS06070	1285927..1286136	-	210	WP_003282211.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11923.68 Da
• Isoelectric Point: 9.8035

>T208975 WP_010799516.1 NZ_CP078087:1280698-1281012 [Stutzerimonas stutzeri]
MIFIETPIFTKRLRDLLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T208975 NZ_CP103768:c3898968-3898462 [Morganella morganii]
TTGAACTTAACGGCACCGGAACCCCTGCAGCAGCATCATGATTTTACCGTTTTTCAGTCAGGTGAGGAGTCCCTTAATCA
TTGGCTGAAAACCCGCGCATTGCAAAATCAGCAATCCGGCGCTTCACGGACATTTGTCGTCTGCCACCATAACCGGATAA
TGGCCTATTATGCATTATCGACAGGTGTTATTACCTGTTCACAGGCGGCGGGGCGCTTCAGACGTAATATGCCTCCGGAA
ATTCCGGTTATTCTGCCGGGACGTCTTGCTGTTGACGAAAGCGTAAAAGGCAGAGGGATCGGACGGGGTCTGATAAAAGA
TGCCGCACTGCGGGTCTTGCAGGCGGCCGGGATTGTGGGTATCCGCGGTATTGTTGTCCGTGCTCTGTCTGATAATGCCA
GACGTTTTTATGAGCATACCGGTTTTATGCCGTCACCGGCTGACCCGATGCTGCTGATGATTACATTACGGGATTTGCAG
CTGGCAACCGGTATCTATTCTGAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10902.47 Da
• Isoelectric Point: 8.7885

>AT208975 WP_010799517.1 NZ_CP078087:1281018-1281308 [Stutzerimonas stutzeri]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKNVIQALSSV

Nucleotide

• Length: 291 bp

>AT208975 NZ_CP103768:c3899279-3898971 [Morganella morganii]
ATGACAAAACACACAGATGAGCAGCATCCTGTGTCCGGCGTGAATATCACCCGTTCAACACTGAATTTACGCATCAAACC
GGAAGATAAAATTCTGATTGATCGCGCAGCGCATGCTGCCGGTAAAAACAGAACCGAGTTCGTACTGGAAGCTGCGCGGC
GTGCCGCTGAAGAAACACTGGCAGATTTACGGGTTATTGATGTCAGTACCGATGTCTATCAGCAGTTTATTACCAGACTG
GATATGGCACCACAGTCCGGTGAAGCGCTGAAGAAGACCATGCTGACGAAGGCGCCGTGGGAGAAGTAA

Structures

Toxin

• AlphaFold DB: N2IK93