Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 188019..188750 | Replicon | plasmid pSA70-3 |
| Accession | NZ_CP078040 | ||
| Organism | Shewanella putrefaciens strain SA70 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | B7D78_RS23385 | Protein ID | WP_086904557.1 |
| Coordinates | 188439..188750 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | B7D78_RS23380 | Protein ID | WP_086904558.1 |
| Coordinates | 188019..188435 (-) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B7D78_RS23325 (KVP08_023325) | 183247..183648 | - | 402 | WP_086904568.1 | S24 family peptidase | - |
| B7D78_RS23330 (KVP08_023330) | 183740..184963 | + | 1224 | WP_028781651.1 | ISL3 family transposase | - |
| B7D78_RS23335 (KVP08_023335) | 185168..185377 | - | 210 | WP_086904567.1 | hypothetical protein | - |
| B7D78_RS23340 (KVP08_023340) | 185405..185653 | - | 249 | WP_086904566.1 | DUF3850 domain-containing protein | - |
| B7D78_RS23345 (KVP08_023345) | 185672..186082 | - | 411 | WP_086904565.1 | hypothetical protein | - |
| B7D78_RS23350 (KVP08_023350) | 186095..186340 | - | 246 | WP_086904564.1 | antirestriction protein ArdR | - |
| B7D78_RS23355 (KVP08_023355) | 186337..186543 | - | 207 | WP_086904563.1 | hypothetical protein | - |
| B7D78_RS23360 (KVP08_023360) | 186563..186778 | - | 216 | WP_086904562.1 | hypothetical protein | - |
| B7D78_RS23365 (KVP08_023365) | 186811..187098 | - | 288 | WP_086904561.1 | hypothetical protein | - |
| B7D78_RS23370 (KVP08_023370) | 187107..187358 | - | 252 | WP_086904560.1 | hypothetical protein | - |
| B7D78_RS23375 (KVP08_023375) | 187565..187864 | + | 300 | WP_086904559.1 | hypothetical protein | - |
| B7D78_RS23380 (KVP08_023380) | 188019..188435 | - | 417 | WP_086904558.1 | transcriptional regulator | Antitoxin |
| B7D78_RS23385 (KVP08_023385) | 188439..188750 | - | 312 | WP_086904557.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| B7D78_RS23390 (KVP08_023390) | 188920..189495 | - | 576 | WP_086904556.1 | SOS response-associated peptidase | - |
| B7D78_RS23395 (KVP08_023395) | 189667..190089 | - | 423 | WP_086904555.1 | hypothetical protein | - |
| B7D78_RS23400 (KVP08_023400) | 190200..190463 | - | 264 | WP_086904554.1 | hypothetical protein | - |
| B7D78_RS23405 (KVP08_023405) | 190498..190812 | - | 315 | WP_086904553.1 | hypothetical protein | - |
| B7D78_RS23410 (KVP08_023410) | 191329..191733 | + | 405 | WP_011790469.1 | Hg(II)-responsive transcriptional regulator | - |
| B7D78_RS23415 (KVP08_023415) | 191813..192160 | + | 348 | WP_011790470.1 | mercuric transporter MerT family protein | - |
| B7D78_RS23420 (KVP08_023420) | 192213..192488 | + | 276 | WP_011790471.1 | mercury resistance system periplasmic binding protein MerP | - |
| B7D78_RS23425 (KVP08_023425) | 192601..193410 | + | 810 | Protein_207 | FAD-dependent oxidoreductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mph(E) / msr(E) / mph(A) / sul1 / qacE / ant(3'')-Ia / blaOXA-10 / blaNDM-1 / qnrA1 / ARR-3 / dfrA14 / qnrVC1 / dfrA15 / aadA10 / aac(6')-Il / tet(C) | - | 1..211155 | 211155 | |
| - | flank | IS/Tn | - | - | 183740..184963 | 1223 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12475.11 Da Isoelectric Point: 9.8965
>T208951 WP_086904557.1 NZ_CP078040:c188750-188439 [Shewanella putrefaciens]
VHVLSRKPFNDAARHYPNQREALVSAYQTLRAGEFRTPNELKQVFPSLDNFKYRDKWWVIDIGGNHLRLIAFIEFRDNRM
YVKHIVPHAEYDDLTDRYRRNKE
VHVLSRKPFNDAARHYPNQREALVSAYQTLRAGEFRTPNELKQVFPSLDNFKYRDKWWVIDIGGNHLRLIAFIEFRDNRM
YVKHIVPHAEYDDLTDRYRRNKE
Download Length: 312 bp
>T208951 NZ_CP103762:1944380-1944634 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Antitoxin
Download Length: 139 a.a. Molecular weight: 15591.69 Da Isoelectric Point: 4.4403
>AT208951 WP_086904558.1 NZ_CP078040:c188435-188019 [Shewanella putrefaciens]
MFPQACHLIREALEMAPFIAEINGQDDYDKALATMDTLIDDYEANKHVINMLSITIERWESSADEFAQFNQAVEEADTGI
ALLKTLMDQYQLGVNDLPELGTKANVSKLLNHTDGKKLTRNHIEALSKRFNLSPAMFF
MFPQACHLIREALEMAPFIAEINGQDDYDKALATMDTLIDDYEANKHVINMLSITIERWESSADEFAQFNQAVEEADTGI
ALLKTLMDQYQLGVNDLPELGTKANVSKLLNHTDGKKLTRNHIEALSKRFNLSPAMFF
Download Length: 417 bp
>AT208951 NZ_CP103762:1944132-1944383 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|