Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4037135..4037732 Replicon chromosome
Accession NZ_CP078033
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag EGK18_RS19735 Protein ID WP_004142563.1
Coordinates 4037415..4037732 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag EGK18_RS19730 Protein ID WP_004142561.1
Coordinates 4037135..4037422 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS19700 (4033215) 4033215..4033463 + 249 WP_002893037.1 DUF1158 domain-containing protein -
EGK18_RS19705 (4033481) 4033481..4033822 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
EGK18_RS19710 (4033853) 4033853..4034968 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
EGK18_RS19715 (4035148) 4035148..4035729 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
EGK18_RS19720 (4035729) 4035729..4036097 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
EGK18_RS19725 (4036217) 4036217..4036867 + 651 Protein_3891 oxygen-insensitive NAD(P)H nitroreductase -
EGK18_RS19730 (4037135) 4037135..4037422 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
EGK18_RS19735 (4037415) 4037415..4037732 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGK18_RS19740 (4037917) 4037917..4038960 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
EGK18_RS19745 (4039627) 4039627..4040493 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
EGK18_RS19750 (4040602) 4040602..4042029 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T208933 WP_004142563.1 NZ_CP078033:c4037732-4037415 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T208933 NZ_CP103758:2615946-2616053 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT208933 WP_004142561.1 NZ_CP078033:c4037422-4037135 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT208933 NZ_CP103758:c2615899-2615833 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References