Detailed information of TA system TA208919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 9228..9813
• Replicon: plasmid pAV_175-4
• Accession: NZ_CP078030
• Organism: Acinetobacter variabilis strain AV_175

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: EGK58_RS16645
• Protein ID: WP_000897307.1
• Coordinates: 9493..9813 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGK58_RS16640
• Protein ID: WP_000369781.1
• Coordinates: 9228..9500 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGK58_RS16625 (EGK58_016625)	5519..6130	+	612	WP_015060246.1	recombinase family protein	-
EGK58_RS16630 (EGK58_016630)	6320..7204	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
EGK58_RS16635 (EGK58_016635)	7260..8735	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
EGK58_RS16640 (EGK58_016640)	9228..9500	-	273	WP_000369781.1	NadS family protein	Antitoxin
EGK58_RS16645 (EGK58_016645)	9493..9813	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGK58_RS16650 (EGK58_016650)	9953..10177	+	225	WP_125279881.1	SMI1/KNR4 family protein	-
EGK58_RS16655 (EGK58_016655)	10199..10582	+	384	WP_001055589.1	transposase	-
EGK58_RS16660 (EGK58_016660)	10579..10914	+	336	WP_219014810.1	IS66 family insertion sequence element accessory protein TnpB	-
EGK58_RS16665 (EGK58_016665)	10989..12593	+	1605	WP_219014809.1	IS66 family transposase	-
EGK58_RS16670 (EGK58_016670)	12651..12890	+	240	WP_267460446.1	SMI1/KNR4 family protein	-
EGK58_RS16675 (EGK58_016675)	12970..13251	-	282	WP_000985609.1	putative addiction module antidote protein	-
EGK58_RS16680 (EGK58_016680)	13252..13554	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGK58_RS16685 (EGK58_016685)	13703..14233	+	531	WP_000172759.1	hypothetical protein	-
EGK58_RS16690 (EGK58_016690)	14322..14636	-	315	WP_004728120.1	BrnA antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / mph(E) / msr(E)	-	1..19843	19843
	-	inside	IScluster/Tn	tet(39) / mph(E) / msr(E)	-	4172..12593	8421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T208919 WP_000897307.1 NZ_CP078030:c9813-9493 [Acinetobacter variabilis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T208919 NZ_CP103757:59851-60129 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT208919 WP_000369781.1 NZ_CP078030:c9500-9228 [Acinetobacter variabilis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT208919 NZ_CP103757:59582-59851 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3