Detailed information of TA system TA208913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 320142..320727
• Replicon: plasmid pAJ_351-2
• Accession: NZ_CP078019
• Organism: Acinetobacter junii strain AJ_351

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: EGT69_RS18005
• Protein ID: WP_000897307.1
• Coordinates: 320407..320727 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGT69_RS18000
• Protein ID: WP_000369781.1
• Coordinates: 320142..320414 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGT69_RS17985 (EGT69_017985)	315746..316630	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
EGT69_RS17990 (EGT69_017990)	316686..318161	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
EGT69_RS17995 (EGT69_017995)	318726..320051	+	1326	WP_125298228.1	ISNCY family transposase	-
EGT69_RS18000 (EGT69_018000)	320142..320414	-	273	WP_000369781.1	NadS family protein	Antitoxin
EGT69_RS18005 (EGT69_018005)	320407..320727	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGT69_RS18010 (EGT69_018010)	320826..321431	-	606	WP_000447788.1	LysE family translocator	-
EGT69_RS18015 (EGT69_018015)	321481..322122	-	642	WP_002042418.1	AraC family transcriptional regulator	-
EGT69_RS18025 (EGT69_018025)	323440..323619	-	180	Protein_318	AraC family ligand binding domain-containing protein	-
EGT69_RS18030 (EGT69_018030)	323793..324499	-	707	Protein_319	IS1 family transposase	-
EGT69_RS18035 (EGT69_018035)	324590..325432	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(X3) / sul2 / aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) / blaNDM-1 / ARR-3 / qacE / sul1 / aph(3')-VIa / blaOXA-58	-	1..332451	332451
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	314883..329600	14717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T208913 WP_000897307.1 NZ_CP078019:c320727-320407 [Acinetobacter junii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T208913 NZ_CP103755:4750721-4751098 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT208913 WP_000369781.1 NZ_CP078019:c320414-320142 [Acinetobacter junii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT208913 NZ_CP103755:4750297-4750674 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3