Detailed information of TA system TA208871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5534649..5535244
• Replicon: chromosome
• Accession: NZ_CP078007
• Organism: Pseudomonas aeruginosa strain FAHZU40

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KUU69_RS25900
• Protein ID: WP_003117425.1
• Coordinates: 5534966..5535244 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU69_RS25895
• Protein ID: WP_003113527.1
• Coordinates: 5534649..5534954 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU69_RS25880 (KUU69_25860)	5529986..5531422	+	1437	WP_079393696.1	HAD-IIIA family hydrolase	-
KUU69_RS25885 (KUU69_25865)	5531426..5532385	+	960	WP_078459576.1	DNA-processing protein DprA	-
KUU69_RS25890 (KUU69_25870)	5532420..5533292	-	873	WP_023122808.1	DUF4868 domain-containing protein	-
KUU69_RS25895 (KUU69_25875)	5534649..5534954	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KUU69_RS25900 (KUU69_25880)	5534966..5535244	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU69_RS30855	5535297..5535425	-	129	Protein_5106	integrase	-
KUU69_RS25905 (KUU69_25885)	5535573..5537801	+	2229	WP_003113525.1	TonB-dependent receptor	-
KUU69_RS25910 (KUU69_25890)	5537871..5538518	-	648	WP_003095021.1	carbonate dehydratase	-
KUU69_RS25915 (KUU69_25895)	5538580..5539818	-	1239	WP_003162396.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T208871 WP_003117425.1 NZ_CP078007:c5535244-5534966 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208871 NZ_CP103745:c3854116-3853718 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT208871 WP_003113527.1 NZ_CP078007:c5534954-5534649 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208871 NZ_CP103745:c3854412-3854119 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5