Detailed information of TA system TA208866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6090453..6091048
• Replicon: chromosome
• Accession: NZ_CP078006
• Organism: Pseudomonas aeruginosa strain NDTH7329

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KUU80_RS28540
• Protein ID: WP_003113526.1
• Coordinates: 6090770..6091048 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU80_RS28535
• Protein ID: WP_003099268.1
• Coordinates: 6090453..6090758 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU80_RS28505 (KUU80_28500)	6085499..6085933	-	435	WP_009875803.1	hypothetical protein	-
KUU80_RS28510 (KUU80_28505)	6086449..6086739	-	291	WP_152959653.1	DUF5447 family protein	-
KUU80_RS28515 (KUU80_28510)	6086915..6087187	-	273	WP_058175553.1	hypothetical protein	-
KUU80_RS28520 (KUU80_28515)	6087634..6087969	+	336	WP_058175552.1	hypothetical protein	-
KUU80_RS28525 (KUU80_28520)	6087972..6088673	-	702	WP_152959654.1	retron Ec48 family effector membrane protein	-
KUU80_RS28530 (KUU80_28525)	6088660..6089835	-	1176	WP_071557852.1	reverse transcriptase family protein	-
KUU80_RS28535 (KUU80_28530)	6090453..6090758	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KUU80_RS28540 (KUU80_28535)	6090770..6091048	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU80_RS33575	6091101..6091229	-	129	Protein_5663	integrase	-
KUU80_RS28545 (KUU80_28540)	6091377..6093605	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU80_RS28550 (KUU80_28545)	6093675..6094322	-	648	WP_003095021.1	carbonate dehydratase	-
KUU80_RS28555 (KUU80_28550)	6094384..6095622	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T208866 WP_003113526.1 NZ_CP078006:c6091048-6090770 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208866 NZ_CP103745:c2466578-2466402 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT208866 WP_003099268.1 NZ_CP078006:c6090758-6090453 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208866 NZ_CP103745:c2466356-2465940 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3