Detailed information of TA system TA208858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5915614..5916209
• Replicon: chromosome
• Accession: NZ_CP078004
• Organism: Pseudomonas aeruginosa strain QZPH16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KUU76_RS27620
• Protein ID: WP_003113526.1
• Coordinates: 5915931..5916209 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU76_RS27615
• Protein ID: WP_003099268.1
• Coordinates: 5915614..5915919 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU76_RS27585 (KUU76_27580)	5910660..5911094	-	435	WP_009875803.1	hypothetical protein	-
KUU76_RS27590 (KUU76_27585)	5911610..5911900	-	291	WP_152959653.1	DUF5447 family protein	-
KUU76_RS27595 (KUU76_27590)	5912076..5912348	-	273	WP_058175553.1	hypothetical protein	-
KUU76_RS27600 (KUU76_27595)	5912795..5913130	+	336	WP_058175552.1	hypothetical protein	-
KUU76_RS27605 (KUU76_27600)	5913133..5913834	-	702	WP_152959654.1	retron Ec48 family effector membrane protein	-
KUU76_RS27610 (KUU76_27605)	5913821..5914996	-	1176	WP_071557852.1	reverse transcriptase family protein	-
KUU76_RS27615 (KUU76_27610)	5915614..5915919	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KUU76_RS27620 (KUU76_27615)	5915931..5916209	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU76_RS32980	5916262..5916390	-	129	Protein_5476	integrase	-
KUU76_RS27625 (KUU76_27620)	5916538..5918766	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU76_RS27630 (KUU76_27625)	5918836..5919483	-	648	WP_003095021.1	carbonate dehydratase	-
KUU76_RS27635 (KUU76_27630)	5919545..5920783	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T208858 WP_003113526.1 NZ_CP078004:c5916209-5915931 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208858 NZ_CP103745:c791982-791605 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT208858 WP_003099268.1 NZ_CP078004:c5915919-5915614 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208858 NZ_CP103745:c792439-792071 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3