Detailed information of TA system TA208817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5532157..5532752
• Replicon: chromosome
• Accession: NZ_CP077988
• Organism: Pseudomonas aeruginosa strain ZPPH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KUU79_RS26030
• Protein ID: WP_003117425.1
• Coordinates: 5532474..5532752 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU79_RS26025
• Protein ID: WP_003113527.1
• Coordinates: 5532157..5532462 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU79_RS25990 (KUU79_25975)	5527301..5527516	-	216	WP_226297985.1	hypothetical protein	-
KUU79_RS25995 (KUU79_25980)	5527509..5527781	-	273	WP_003115921.1	hypothetical protein	-
KUU79_RS26000 (KUU79_25985)	5528228..5528563	+	336	WP_116802237.1	hypothetical protein	-
KUU79_RS26005 (KUU79_25990)	5528608..5530203	-	1596	WP_147314246.1	DUF2326 domain-containing protein	-
KUU79_RS26010 (KUU79_25995)	5530432..5530926	-	495	WP_147314247.1	hypothetical protein	-
KUU79_RS26015 (KUU79_26000)	5530926..5531102	-	177	WP_165567202.1	hypothetical protein	-
KUU79_RS26020 (KUU79_26005)	5531443..5531820	-	378	WP_116802239.1	hypothetical protein	-
KUU79_RS26025 (KUU79_26010)	5532157..5532462	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KUU79_RS26030 (KUU79_26015)	5532474..5532752	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU79_RS32355	5532805..5532933	-	129	Protein_5162	integrase	-
KUU79_RS26035 (KUU79_26020)	5533081..5535309	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU79_RS26040 (KUU79_26025)	5535379..5536026	-	648	WP_003095021.1	carbonate dehydratase	-
KUU79_RS26045 (KUU79_26030)	5536088..5537326	-	1239	WP_031685043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T208817 WP_003117425.1 NZ_CP077988:c5532752-5532474 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208817 NZ_CP103739:c3026489-3026235 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT208817 WP_003113527.1 NZ_CP077988:c5532462-5532157 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208817 NZ_CP103739:c3026737-3026486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5