Detailed information of TA system TA208809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5608184..5608779
• Replicon: chromosome
• Accession: NZ_CP077985
• Organism: Pseudomonas aeruginosa strain ZPPH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KUU77_RS26430
• Protein ID: WP_003117425.1
• Coordinates: 5608501..5608779 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU77_RS26425
• Protein ID: WP_003113527.1
• Coordinates: 5608184..5608489 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU77_RS26390 (KUU77_26375)	5603328..5603543	-	216	WP_226297985.1	hypothetical protein	-
KUU77_RS26395 (KUU77_26380)	5603536..5603808	-	273	WP_003115921.1	hypothetical protein	-
KUU77_RS26400 (KUU77_26385)	5604255..5604590	+	336	WP_116802237.1	hypothetical protein	-
KUU77_RS26405 (KUU77_26390)	5604635..5606230	-	1596	WP_147314246.1	DUF2326 domain-containing protein	-
KUU77_RS26410 (KUU77_26395)	5606459..5606953	-	495	WP_147314247.1	hypothetical protein	-
KUU77_RS26415 (KUU77_26400)	5606953..5607129	-	177	WP_165567202.1	hypothetical protein	-
KUU77_RS26420 (KUU77_26405)	5607470..5607847	-	378	WP_116802239.1	hypothetical protein	-
KUU77_RS26425 (KUU77_26410)	5608184..5608489	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KUU77_RS26430 (KUU77_26415)	5608501..5608779	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU77_RS32135	5608832..5608960	-	129	Protein_5237	integrase	-
KUU77_RS26435 (KUU77_26420)	5609108..5611336	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU77_RS26440 (KUU77_26425)	5611406..5612053	-	648	WP_003095021.1	carbonate dehydratase	-
KUU77_RS26445 (KUU77_26430)	5612115..5613353	-	1239	WP_031685043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T208809 WP_003117425.1 NZ_CP077985:c5608779-5608501 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208809 NZ_CP103739:c1332819-1332601 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT208809 WP_003113527.1 NZ_CP077985:c5608489-5608184 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208809 NZ_CP103739:c1333219-1332845 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5