Detailed information of TA system TA208803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5362898..5363493
• Replicon: chromosome
• Accession: NZ_CP077981
• Organism: Pseudomonas aeruginosa strain ZPPH14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KUU73_RS24695
• Protein ID: WP_003113526.1
• Coordinates: 5363215..5363493 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU73_RS24690
• Protein ID: WP_124182647.1
• Coordinates: 5362898..5363203 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU73_RS24655 (KUU73_24650)	5358047..5358895	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KUU73_RS24665 (KUU73_24660)	5359062..5360003	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KUU73_RS24670 (KUU73_24665)	5360120..5360734	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KUU73_RS24675 (KUU73_24670)	5360776..5361360	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KUU73_RS24680 (KUU73_24675)	5361401..5362501	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KUU73_RS24690 (KUU73_24685)	5362898..5363203	-	306	WP_124182647.1	HigA family addiction module antitoxin	Antitoxin
KUU73_RS24695 (KUU73_24690)	5363215..5363493	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU73_RS29910	5363546..5363674	-	129	Protein_4893	integrase	-
KUU73_RS24700 (KUU73_24695)	5363822..5366050	+	2229	WP_031633241.1	TonB-dependent receptor	-
KUU73_RS24705 (KUU73_24700)	5366120..5366767	-	648	WP_003095021.1	carbonate dehydratase	-
KUU73_RS24710 (KUU73_24705)	5366829..5368067	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T208803 WP_003113526.1 NZ_CP077981:c5363493-5363215 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208803 NZ_CP103732:c110454-109972 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11241.85 Da
• Isoelectric Point: 5.0747

>AT208803 WP_124182647.1 NZ_CP077981:c5363203-5362898 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAVNGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208803 NZ_CP103732:c110723-110445 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3