Detailed information of TA system TA208794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5569819..5570414
• Replicon: chromosome
• Accession: NZ_CP077977
• Organism: Pseudomonas aeruginosa strain ZPPH29

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KUU70_RS26200
• Protein ID: WP_003113526.1
• Coordinates: 5570136..5570414 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU70_RS26195
• Protein ID: WP_003099268.1
• Coordinates: 5569819..5570124 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU70_RS26160 (KUU70_26155)	5564959..5565807	+	849	WP_023115601.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KUU70_RS26170 (KUU70_26165)	5565974..5566915	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KUU70_RS26175 (KUU70_26170)	5567032..5567646	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KUU70_RS26180 (KUU70_26175)	5567688..5568272	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KUU70_RS26185 (KUU70_26180)	5568313..5569413	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KUU70_RS26195 (KUU70_26190)	5569819..5570124	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KUU70_RS26200 (KUU70_26195)	5570136..5570414	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU70_RS33690	5570467..5570595	-	129	Protein_5186	integrase	-
KUU70_RS26205 (KUU70_26200)	5570743..5572971	+	2229	WP_031633241.1	TonB-dependent receptor	-
KUU70_RS26210 (KUU70_26205)	5573041..5573688	-	648	WP_003095021.1	carbonate dehydratase	-
KUU70_RS26215 (KUU70_26210)	5573750..5574988	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T208794 WP_003113526.1 NZ_CP077977:c5570414-5570136 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208794 NZ_CP103731:c3977976-3977659 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT208794 WP_003099268.1 NZ_CP077977:c5570124-5569819 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208794 NZ_CP103731:c3977666-3977379 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: V6ALY3