Detailed information of TA system TA208788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5463932..5464527
• Replicon: chromosome
• Accession: NZ_CP077971
• Organism: Pseudomonas aeruginosa strain ZPPH33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KUU78_RS25715
• Protein ID: WP_003117425.1
• Coordinates: 5464249..5464527 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUU78_RS25710
• Protein ID: WP_003113527.1
• Coordinates: 5463932..5464237 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU78_RS25675 (KUU78_25660)	5459076..5459291	-	216	WP_226297985.1	hypothetical protein	-
KUU78_RS25680 (KUU78_25665)	5459284..5459556	-	273	WP_003115921.1	hypothetical protein	-
KUU78_RS25685 (KUU78_25670)	5460003..5460338	+	336	WP_116802237.1	hypothetical protein	-
KUU78_RS25690 (KUU78_25675)	5460383..5461978	-	1596	WP_222822315.1	DUF2326 domain-containing protein	-
KUU78_RS25695 (KUU78_25680)	5462207..5462701	-	495	WP_147314247.1	hypothetical protein	-
KUU78_RS25700 (KUU78_25685)	5462701..5462877	-	177	WP_165567202.1	hypothetical protein	-
KUU78_RS25705 (KUU78_25690)	5463218..5463595	-	378	WP_116802239.1	hypothetical protein	-
KUU78_RS25710 (KUU78_25695)	5463932..5464237	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KUU78_RS25715 (KUU78_25700)	5464249..5464527	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU78_RS32300	5464580..5464708	-	129	Protein_5096	integrase	-
KUU78_RS25720 (KUU78_25705)	5464856..5467084	+	2229	WP_003099265.1	TonB-dependent receptor	-
KUU78_RS25725 (KUU78_25710)	5467154..5467801	-	648	WP_003095021.1	carbonate dehydratase	-
KUU78_RS25730 (KUU78_25715)	5467863..5469101	-	1239	WP_031685043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T208788 WP_003117425.1 NZ_CP077971:c5464527-5464249 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T208788 NZ_CP103730:c144541-144059 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT208788 WP_003113527.1 NZ_CP077971:c5464237-5463932 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT208788 NZ_CP103730:c144795-144529 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5