Detailed information of TA system TA208740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13610..14267
• Replicon: plasmid p6Pmi283-NDM
• Accession: NZ_CP077964
• Organism: Proteus mirabilis strain 6Pmi283

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KPK64_RS18125
• Protein ID: WP_000270043.1
• Coordinates: 13917..14267 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPK64_RS18120
• Protein ID: WP_000124640.1
• Coordinates: 13610..13912 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK64_RS18075 (KPK64_03640)	9235..9663	+	429	WP_000591074.1	hypothetical protein	-
KPK64_RS18080 (KPK64_03641)	9720..10079	+	360	WP_000422768.1	hypothetical protein	-
KPK64_RS18085 (KPK64_03642)	10079..10525	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
KPK64_RS18090 (KPK64_03643)	10522..11040	+	519	WP_000210756.1	nitrite reductase	-
KPK64_RS18095 (KPK64_03644)	11040..11270	+	231	WP_000972663.1	hypothetical protein	-
KPK64_RS18100 (KPK64_03645)	11257..12114	+	858	WP_001167032.1	hypothetical protein	-
KPK64_RS18105 (KPK64_03646)	12345..12872	+	528	WP_001236377.1	thermonuclease family protein	-
KPK64_RS18110 (KPK64_03647)	12930..13202	+	273	WP_001043047.1	HU family DNA-binding protein	-
KPK64_RS18115	13290..13583	+	294	WP_001239997.1	chromosome segregation protein ParM	-
KPK64_RS18120 (KPK64_03648)	13610..13912	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KPK64_RS18125 (KPK64_03649)	13917..14267	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPK64_RS18130 (KPK64_03650)	14430..14978	+	549	WP_001061574.1	transcriptional regulator	-
KPK64_RS18135 (KPK64_03652)	15319..15513	+	195	WP_000343597.1	hypothetical protein	-
KPK64_RS18140 (KPK64_03653)	15524..15895	+	372	WP_000516916.1	hypothetical protein	-
KPK64_RS18145 (KPK64_03654)	15888..16358	+	471	WP_001281821.1	hypothetical protein	-
KPK64_RS18150 (KPK64_03655)	16373..16708	-	336	WP_000683476.1	hypothetical protein	-
KPK64_RS18155 (KPK64_03656)	16805..17293	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KPK64_RS18160 (KPK64_03657)	17296..17793	+	498	WP_000062185.1	hypothetical protein	-
KPK64_RS18165 (KPK64_03658)	18008..18712	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KPK64_RS18170	18777..18956	-	180	Protein_32	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / aac(6')-Ian / aph(6)-Id / aph(3'')-Ib / qnrA1 / aph(3')-VI / blaNDM-1	-	1..211006	211006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T208740 WP_000270043.1 NZ_CP077964:c14267-13917 [Proteus mirabilis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T208740 NZ_CP103718:c3140190-3139936 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT208740 WP_000124640.1 NZ_CP077964:c13912-13610 [Proteus mirabilis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT208740 NZ_CP103718:c3140438-3140187 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9