Detailed information of TA system TA208639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 693754..694202
• Replicon: chromosome
• Accession: NZ_CP077924
• Organism: Staphylococcus aureus strain 200

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A853P973
• Locus tag: KU536_RS03650
• Protein ID: WP_000387656.1
• Coordinates: 693900..694202 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	693754..693820 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU536_RS03620 (688855)	688855..692535	+	3681	WP_000582140.1	phage tail spike protein	-
KU536_RS03625 (692522)	692522..692674	+	153	WP_001181555.1	hypothetical protein	-
KU536_RS03630 (692721)	692721..693008	+	288	WP_001040257.1	hypothetical protein	-
KU536_RS03635 (693066)	693066..693362	+	297	WP_000539688.1	DUF2951 domain-containing protein	-
KU536_RS03640 (693594)	693594..693701	+	108	WP_000253688.1	putative holin-like toxin	-
- (693754)	693754..693820	-	67	NuclAT_0	-	Antitoxin
- (693754)	693754..693820	-	67	NuclAT_0	-	Antitoxin
- (693754)	693754..693820	-	67	NuclAT_0	-	Antitoxin
- (693754)	693754..693820	-	67	NuclAT_0	-	Antitoxin
KU536_RS03645 (693745)	693745..693849	-	105	Protein_714	hypothetical protein	-
KU536_RS03650 (693900)	693900..694202	+	303	WP_000387656.1	phage holin	Toxin
KU536_RS03655 (694214)	694214..695668	+	1455	WP_000930259.1	N-acetylmuramoyl-L-alanine amidase	-
KU536_RS03660 (695763)	695763..696212	-	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
KU536_RS03665 (696897)	696897..697247	+	351	WP_000702263.1	complement inhibitor SCIN-A	-
KU536_RS03670 (697300)	697300..697560	-	261	WP_001791826.1	hypothetical protein	-
KU536_RS03675 (697871)	697871..698050	-	180	WP_000669789.1	hypothetical protein	-
KU536_RS03680 (698422)	698422..698619	-	198	WP_000239614.1	phospholipase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / hlb / chp / scn	646277..697247	50970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11177.94 Da
• Isoelectric Point: 9.6587

>T208639 WP_000387656.1 NZ_CP077924:693900-694202 [Staphylococcus aureus]
MEAKVMARYVVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTTYKDNPTSQEGRWANQKLKKYKAESKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T208639 NZ_CP103697:718602-718704 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 67 bp

>AT208639 NZ_CP077924:c693820-693754 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Structures

Toxin

• AlphaFold DB: A0A853P973

Antitoxin