Detailed information of TA system TA208622

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 641097..641545
• Replicon: chromosome
• Accession: NZ_CP077923
• Organism: Staphylococcus aureus strain 201

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A853P973
• Locus tag: KU534_RS03380
• Protein ID: WP_000387656.1
• Coordinates: 641243..641545 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	641097..641163 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU534_RS03350 (636198)	636198..639878	+	3681	WP_000582140.1	phage tail spike protein	-
KU534_RS03355 (639865)	639865..640017	+	153	WP_001181555.1	hypothetical protein	-
KU534_RS03360 (640064)	640064..640351	+	288	WP_001040257.1	hypothetical protein	-
KU534_RS03365 (640409)	640409..640705	+	297	WP_000539688.1	DUF2951 domain-containing protein	-
KU534_RS03370 (640937)	640937..641044	+	108	WP_000253688.1	putative holin-like toxin	-
- (641097)	641097..641163	-	67	NuclAT_0	-	Antitoxin
- (641097)	641097..641163	-	67	NuclAT_0	-	Antitoxin
- (641097)	641097..641163	-	67	NuclAT_0	-	Antitoxin
- (641097)	641097..641163	-	67	NuclAT_0	-	Antitoxin
KU534_RS03375 (641088)	641088..641192	-	105	Protein_660	hypothetical protein	-
KU534_RS03380 (641243)	641243..641545	+	303	WP_000387656.1	phage holin	Toxin
KU534_RS03385 (641557)	641557..643011	+	1455	WP_000930259.1	N-acetylmuramoyl-L-alanine amidase	-
KU534_RS03390 (643106)	643106..643555	-	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
KU534_RS03395 (644240)	644240..644590	+	351	WP_000702263.1	complement inhibitor SCIN-A	-
KU534_RS03400 (644643)	644643..644903	-	261	WP_001791826.1	hypothetical protein	-
KU534_RS03405 (645214)	645214..645393	-	180	WP_000669789.1	hypothetical protein	-
KU534_RS03410 (645765)	645765..645962	-	198	WP_000239614.1	phospholipase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / hlb / chp / scn	593620..644590	50970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11177.94 Da
• Isoelectric Point: 9.6587

>T208622 WP_000387656.1 NZ_CP077923:641243-641545 [Staphylococcus aureus]
MEAKVMARYVVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTTYKDNPTSQEGRWANQKLKKYKAESKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T208622 NZ_CP103694:719907-720203 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 67 bp

>AT208622 NZ_CP077923:c641163-641097 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Structures

Toxin

• AlphaFold DB: A0A853P973

Antitoxin