Detailed information of TA system TA020861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 508848..509350
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q6DA20
• Locus tag: ECA_RS02255
• Protein ID: WP_011092065.1
• Coordinates: 508848..509102 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q6DA19
• Locus tag: ECA_RS02260
• Protein ID: WP_011092066.1
• Coordinates: 509099..509350 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS02235	504022..505284	-	1263	WP_011092061.1	L-rhamnose isomerase	-
ECA_RS02240	505281..506771	-	1491	WP_011092062.1	rhamnulokinase	-
ECA_RS02245	507088..507927	+	840	WP_011092063.1	HTH-type transcriptional activator RhaS	-
ECA_RS02250	507952..508806	+	855	WP_011092064.1	helix-turn-helix domain-containing protein	-
ECA_RS02255	508848..509102	-	255	WP_011092065.1	Txe/YoeB family addiction module toxin	Toxin
ECA_RS02260	509099..509350	-	252	WP_011092066.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
ECA_RS02265	509425..511134	-	1710	WP_011092067.1	ABC transporter ATP-binding protein	-
ECA_RS02270	511131..511997	-	867	WP_011092068.1	ABC transporter permease	-
ECA_RS02275	511990..512949	-	960	WP_011092069.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10141.67 Da
• Isoelectric Point: 8.8560

>T20861 WP_011092065.1 NC_004547:c509102-508848 [Pectobacterium atrosepticum SCRI1043]
VKLIWSEEAWEDYLYWQDIDKRTVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEAHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T20861 NC_004547:c509102-508848 [Pectobacterium atrosepticum SCRI1043]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGAACGGTCAAAAAGAT
CAATGAATTAATAAAAGAGACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGCACATCGTCTGGTGTATGCCATCACAGATGATGCCATGATGATCGCCGCATGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9397.66 Da
• Isoelectric Point: 4.6119

>AT20861 WP_011092066.1 NC_004547:c509350-509099 [Pectobacterium atrosepticum SCRI1043]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNSEERD
IIE

Nucleotide

• Length: 252 bp

>AT20861 NC_004547:c509350-509099 [Pectobacterium atrosepticum SCRI1043]
ATGCGTACAATTAGCTACAGCGAAGCCCGTCAAAATCTATCAGCGACAATGATGAAAACCGTCGAAGATCGTGCTCCCAT
TCTGATCACTCGACAGAATGGTGAGGCCTGCGTGCTTATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACAGCATATT
TACTGCGTTCACCAGCAAATGCAAAAAGGCTGATGAATTCAATTGAAAGCCTTAAGGCTGGCAATAGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q6DA20

Antitoxin

• AlphaFold DB: Q6DA19