Detailed information of TA system TA208589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2263892..2264409
• Replicon: chromosome
• Accession: NZ_CP077921
• Organism: Staphylococcus aureus strain 276

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: KU530_RS11010
• Protein ID: WP_000113262.1
• Coordinates: 2264143..2264409 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU530_RS11005
• Protein ID: WP_000584499.1
• Coordinates: 2263892..2264143 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU530_RS10975 (KU530_10935)	2259590..2260309	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU530_RS10980 (KU530_10940)	2260306..2261037	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU530_RS10985 (KU530_10945)	2261134..2262384	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
KU530_RS10990 (KU530_10950)	2262639..2263001	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KU530_RS10995 (KU530_10955)	2263020..2263619	+	600	WP_000162810.1	DsbA family protein	-
KU530_RS11000 (KU530_10960)	2263647..2263727	+	81	WP_223197974.1	hypothetical protein	-
KU530_RS11005 (KU530_10965)	2263892..2264143	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU530_RS11010 (KU530_10970)	2264143..2264409	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
KU530_RS11015	2264444..2264515	+	72	WP_223200558.1	hypothetical protein	-
KU530_RS11020 (KU530_10975)	2264703..2266253	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU530_RS11025 (KU530_10980)	2266439..2266906	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
KU530_RS11030 (KU530_10985)	2266993..2267175	+	183	WP_000079142.1	hypothetical protein	-
KU530_RS11035 (KU530_10990)	2267392..2268216	+	825	WP_225806570.1	formate/nitrite transporter family protein	-
KU530_RS11040 (KU530_10995)	2268450..2268986	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T208589 WP_000113262.1 NZ_CP077921:2264143-2264409 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208589 NZ_CP103680:214130-214417 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208589 WP_000584499.1 NZ_CP077921:2263892-2264143 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208589 NZ_CP103680:213891-214130 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60