Detailed information of TA system TA208576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1563008..1563525
• Replicon: chromosome
• Accession: NZ_CP077920
• Organism: Staphylococcus aureus strain 277

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: KU525_RS07605
• Protein ID: WP_000113262.1
• Coordinates: 1563259..1563525 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU525_RS07600
• Protein ID: WP_000584499.1
• Coordinates: 1563008..1563259 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU525_RS07570 (1558706)	1558706..1559425	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU525_RS07575 (1559422)	1559422..1560153	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU525_RS07580 (1560250)	1560250..1561500	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
KU525_RS07585 (1561755)	1561755..1562117	+	363	WP_000819840.1	DUF4467 domain-containing protein	-
KU525_RS07590 (1562136)	1562136..1562735	+	600	WP_000162810.1	DsbA family protein	-
KU525_RS07595 (1562763)	1562763..1562843	+	81	WP_223197974.1	hypothetical protein	-
KU525_RS07600 (1563008)	1563008..1563259	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU525_RS07605 (1563259)	1563259..1563525	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
KU525_RS07610 (1563560)	1563560..1563631	+	72	Protein_1498	hypothetical protein	-
KU525_RS07615 (1563820)	1563820..1565373	+	1554	WP_000727769.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU525_RS07620 (1565559)	1565559..1566026	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
KU525_RS07625 (1566110)	1566110..1566295	+	186	WP_000230293.1	hypothetical protein	-
KU525_RS07630 (1566511)	1566511..1567335	+	825	WP_000594629.1	formate/nitrite transporter family protein	-
KU525_RS07635 (1567569)	1567569..1568105	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T208576 WP_000113262.1 NZ_CP077920:1563259-1563525 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208576 NZ_CP103679:644221-644508 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208576 WP_000584499.1 NZ_CP077920:1563008-1563259 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208576 NZ_CP103679:644495-644797 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGCGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60