Detailed information of TA system TA020856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3325314..3325915
• Replicon: chromosome
• Accession: NC_004463
• Organism: Bradyrhizobium diazoefficiens USDA 110

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: BJA_RS14925
• Protein ID: WP_011085796.1
• Coordinates: 3325634..3325915 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BJA_RS14920
• Protein ID: WP_011085795.1
• Coordinates: 3325314..3325622 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJA_RS14905 (blr3008)	3320560..3323148	+	2589	WP_011085792.1	MMPL family transporter	-
BJA_RS14910 (bll3009)	3323116..3323772	-	657	WP_038965759.1	DUF2147 domain-containing protein	-
BJA_RS14915 (blr3010)	3323914..3325305	+	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
BJA_RS14920 (bll3011)	3325314..3325622	-	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
BJA_RS14925 (bsl3012)	3325634..3325915	-	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJA_RS14930 (bll3013)	3325987..3326754	-	768	WP_011085797.1	anti-sigma factor	-
BJA_RS14935 (bll3014)	3326751..3327299	-	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
BJA_RS14940 (bll3015)	3327427..3327762	-	336	WP_011085799.1	cupredoxin family copper-binding protein	-
BJA_RS14945 (bll3016)	3327788..3328732	-	945	WP_011085800.1	metallophosphoesterase	-
BJA_RS14950 (blr3017)	3328912..3329778	+	867	WP_011085801.1	helix-turn-helix transcriptional regulator	-
BJA_RS14955 (bll3018)	3329801..3330124	-	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
BJA_RS14960 (bll3019)	3330170..3330532	-	363	WP_011085803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T20856 WP_011085796.1 NC_004463:c3325915-3325634 [Bradyrhizobium diazoefficiens USDA 110]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T20856 NC_004463:c3325915-3325634 [Bradyrhizobium diazoefficiens USDA 110]
GTGATCAGAACGTTTCGGGACAAAACGACTGAGGCAGTCTTCGACGGAGAAAGTCCCAAAGGCTTTCCAGCCGATCTCGT
GAAGGTTGCACGCCGGAAACTGCGTTATCTGCACGCTGCCGGCGAGCTCGGCGATCTAAGGGCGCCGCCCGGCAACCGTC
TGGAGGCCCTGAGCGGCAATCGCAAGGGACAGCACTCGATCAGGATCAATGATCAGTTCCGAGTGTGCTTCATCTGGACA
CCGCAAGGGCCCGTCGAGGTCGAGATTGTGGACTATCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT20856 WP_011085795.1 NC_004463:c3325622-3325314 [Bradyrhizobium diazoefficiens USDA 110]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT20856 NC_004463:c3325622-3325314 [Bradyrhizobium diazoefficiens USDA 110]
ATGGCCAAAAAACTCAAGCCCATGCACCCCGGTGAGGTTCTACGGGAGGAGTTTCTAGTCCCACTGAACATGTCCGCTGG
CGCGCTCGCCAAGACGTGCGGCTTGCCGCGCACGCGCATCGAGCGGATCGCGAATGAGCAGACCGGAATCACCGCCGACA
CCGCGCTTCGACTGGCCAAGGCGCTCGGCACAACGGCCGAACTCTGGCTCAATCTTCAGAATGATTATGACATCCAGATC
GCGAAGCGCGATCTCGGCAAGGCACTTGACCGCATCGAGACCGTGAACAGGCCGCAAGCGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2