Detailed information of TA system TA208523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2188029..2188546
• Replicon: chromosome
• Accession: NZ_CP077915
• Organism: Staphylococcus aureus strain 282

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: KU535_RS10495
• Protein ID: WP_000113262.1
• Coordinates: 2188280..2188546 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU535_RS10490
• Protein ID: WP_000584499.1
• Coordinates: 2188029..2188280 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU535_RS10460 (KU535_10415)	2183727..2184446	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU535_RS10465 (KU535_10420)	2184443..2185174	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU535_RS10470 (KU535_10425)	2185271..2186521	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
KU535_RS10475 (KU535_10430)	2186776..2187138	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KU535_RS10480 (KU535_10435)	2187157..2187756	+	600	WP_000162810.1	DsbA family protein	-
KU535_RS10485 (KU535_10440)	2187784..2187864	+	81	WP_223197974.1	hypothetical protein	-
KU535_RS10490 (KU535_10445)	2188029..2188280	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU535_RS10495 (KU535_10450)	2188280..2188546	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
KU535_RS10500	2188581..2188652	+	72	WP_223200558.1	hypothetical protein	-
KU535_RS10505 (KU535_10455)	2188840..2190390	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU535_RS10510 (KU535_10460)	2190576..2191043	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
KU535_RS10515 (KU535_10465)	2191130..2191312	+	183	WP_000079142.1	hypothetical protein	-
KU535_RS10520 (KU535_10470)	2191529..2192353	+	825	WP_225806570.1	formate/nitrite transporter family protein	-
KU535_RS10525 (KU535_10475)	2192587..2193123	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T208523 WP_000113262.1 NZ_CP077915:2188280-2188546 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208523 NZ_CP103672:c4345112-4344828 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208523 WP_000584499.1 NZ_CP077915:2188029-2188280 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208523 NZ_CP103672:c4345348-4345115 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60