Detailed information of TA system TA020836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4601742..4602344
• Replicon: chromosome
• Accession: NC_004431
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: C_RS22860
• Protein ID: WP_000897302.1
• Coordinates: 4601742..4602053 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C_RS22865
• Protein ID: WP_000356397.1
• Coordinates: 4602054..4602344 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C_RS22835	4597655..4598254	+	600	WP_001443191.1	glucose-1-phosphatase	-
C_RS22840	4598248..4599120	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C_RS22845	4599117..4599554	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C_RS22850	4599599..4600540	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C_RS22855	4600604..4601512	-	909	WP_001305059.1	alpha/beta hydrolase	-
C_RS22860	4601742..4602053	+	312	WP_000897302.1	hypothetical protein	Toxin
C_RS22865	4602054..4602344	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C_RS22875	4602703..4602981	+	279	WP_001296612.1	hypothetical protein	-
C_RS22880	4603377..4603595	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
C_RS22885	4603811..4604740	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
C_RS22890	4604737..4605372	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C_RS22895	4605369..4606271	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T20836 WP_000897302.1 NC_004431:4601742-4602053 [Escherichia coli CFT073]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T20836 NC_004431:4601742-4602053 [Escherichia coli CFT073]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT20836 WP_000356397.1 NC_004431:4602054-4602344 [Escherichia coli CFT073]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT20836 NC_004431:4602054-4602344 [Escherichia coli CFT073]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416