Detailed information of TA system TA020834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3714321..3715120
• Replicon: chromosome
• Accession: NC_004431
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: C_RS18435
• Protein ID: WP_000347252.1
• Coordinates: 3714656..3715120 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: C_RS18430
• Protein ID: WP_001296435.1
• Coordinates: 3714321..3714656 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C_RS18415	3710106..3710876	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
C_RS18420	3710892..3712226	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
C_RS18425	3712601..3714172	+	1572	WP_001273940.1	galactarate dehydratase	-
C_RS18430	3714321..3714656	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C_RS18435	3714656..3715120	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C_RS18440	3715175..3715984	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C_RS18445	3716233..3717513	+	1281	WP_000681928.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C_RS18450	3717536..3718009	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C_RS18455	3718020..3718799	+	780	WP_000406224.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C_RS18460	3718789..3719667	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C_RS18465	3719685..3720119	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T20834 WP_000347252.1 NC_004431:3714656-3715120 [Escherichia coli CFT073]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T20834 NC_004431:3714656-3715120 [Escherichia coli CFT073]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT20834 WP_001296435.1 NC_004431:3714321-3714656 [Escherichia coli CFT073]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT20834 NC_004431:3714321-3714656 [Escherichia coli CFT073]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5