Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3121577..3122304 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag C_RS15560 Protein ID WP_000547555.1
Coordinates 3121993..3122304 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag C_RS15555 Protein ID WP_000126293.1
Coordinates 3121577..3121996 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS15540 3117740..3119992 - 2253 WP_001107659.1 carbamoyltransferase HypF -
C_RS15545 3120145..3120672 - 528 WP_001078777.1 electron transport protein HydN -
C_RS15550 3121057..3121485 + 429 WP_000536064.1 hypothetical protein -
C_RS15555 3121577..3121996 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
C_RS15560 3121993..3122304 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
C_RS15565 3122467..3123222 - 756 WP_001305268.1 hypothetical protein -
C_RS15570 3123265..3123735 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
C_RS15575 3123728..3124138 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
C_RS15580 3124135..3124902 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
C_RS15585 3124902..3125444 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
C_RS15590 3125454..3127163 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T20831 WP_000547555.1 NC_004431:c3122304-3121993 [Escherichia coli CFT073]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T20831 NC_004431:c3122304-3121993 [Escherichia coli CFT073]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT20831 WP_000126293.1 NC_004431:c3121996-3121577 [Escherichia coli CFT073]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT20831 NC_004431:c3121996-3121577 [Escherichia coli CFT073]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References