Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2377268..2377770 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag C_RS12070 Protein ID WP_000767822.1
Coordinates 2377268..2377522 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C_RS12075 Protein ID WP_001259255.1
Coordinates 2377519..2377770 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS12055 2373761..2375119 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
C_RS29335 2375109..2375171 - 63 WP_010723108.1 membrane protein YoeI -
C_RS12060 2375386..2376315 - 930 WP_000803366.1 LysR family transcriptional regulator -
C_RS12065 2376361..2377185 - 825 WP_000754733.1 SDR family oxidoreductase -
C_RS12070 2377268..2377522 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C_RS12075 2377519..2377770 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C_RS27185 2378053..2378103 + 51 WP_001364200.1 his operon leader peptide -
C_RS12080 2378249..2379148 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C_RS12085 2379154..2380458 + 1305 WP_001297912.1 histidinol dehydrogenase -
C_RS12090 2380455..2381525 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
C_RS12095 2381525..2382592 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T20830 WP_000767822.1 NC_004431:c2377522-2377268 [Escherichia coli CFT073]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T20830 NC_004431:c2377522-2377268 [Escherichia coli CFT073]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT20830 WP_001259255.1 NC_004431:c2377770-2377519 [Escherichia coli CFT073]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT20830 NC_004431:c2377770-2377519 [Escherichia coli CFT073]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References