Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1745044..1745606 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag C_RS08965 Protein ID WP_000605675.1
Coordinates 1745328..1745606 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C_RS08960 Protein ID WP_000781370.1
Coordinates 1745044..1745328 (-) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS08945 1740228..1743275 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
C_RS08950 1743288..1744172 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
C_RS08955 1744165..1744818 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
C_RS08960 1745044..1745328 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C_RS08965 1745328..1745606 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C_RS08970 1745792..1746802 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
C_RS08975 1746936..1748633 - 1698 WP_000433460.1 malate dehydrogenase -
C_RS08980 1748790..1748927 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C_RS08985 1749029..1749244 - 216 WP_000495766.1 biofilm-dependent modulation protein -
C_RS08990 1749589..1750020 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-92)

Antitoxin

(1-88)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T20820 WP_000605675.1 NC_004431:c1745606-1745328 [Escherichia coli CFT073]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T20820 NC_004431:c1745606-1745328 [Escherichia coli CFT073]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT20820 WP_000781370.1 NC_004431:c1745328-1745044 [Escherichia coli CFT073]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT20820 NC_004431:c1745328-1745044 [Escherichia coli CFT073]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1Q1V8


Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References