Detailed information of TA system TA208173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2244465..2244982
• Replicon: chromosome
• Accession: NZ_CP077857
• Organism: Staphylococcus aureus strain L4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: KU533_RS10930
• Protein ID: WP_000113265.1
• Coordinates: 2244716..2244982 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU533_RS10925
• Protein ID: WP_000584499.1
• Coordinates: 2244465..2244716 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU533_RS10895 (2240163)	2240163..2240882	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU533_RS10900 (2240879)	2240879..2241610	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU533_RS10905 (2241707)	2241707..2242957	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
KU533_RS10910 (2243212)	2243212..2243574	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KU533_RS10915 (2243593)	2243593..2244192	+	600	WP_000162810.1	DsbA family protein	-
KU533_RS10920 (2244220)	2244220..2244300	+	81	WP_223197974.1	hypothetical protein	-
KU533_RS10925 (2244465)	2244465..2244716	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU533_RS10930 (2244716)	2244716..2244982	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
KU533_RS10935 (2245017)	2245017..2245088	+	72	Protein_2120	hypothetical protein	-
KU533_RS10940 (2245277)	2245277..2246824	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU533_RS10945 (2247010)	2247010..2247477	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
KU533_RS10950 (2247561)	2247561..2247743	+	183	WP_000230292.1	hypothetical protein	-
KU533_RS10955 (2247960)	2247960..2248784	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
KU533_RS10960 (2249018)	2249018..2249554	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T208173 WP_000113265.1 NZ_CP077857:2244716-2244982 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208173 NZ_CP103586:c5310798-5310415 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208173 WP_000584499.1 NZ_CP077857:2244465-2244716 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208173 NZ_CP103586:c5311040-5310798 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60