Detailed information of TA system TA208155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2232418..2232935
• Replicon: chromosome
• Accession: NZ_CP077856
• Organism: Staphylococcus aureus strain L5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: KU529_RS10760
• Protein ID: WP_000113265.1
• Coordinates: 2232669..2232935 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU529_RS10755
• Protein ID: WP_000584499.1
• Coordinates: 2232418..2232669 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU529_RS10725 (2228116)	2228116..2228835	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU529_RS10730 (2228832)	2228832..2229563	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU529_RS10735 (2229660)	2229660..2230910	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
KU529_RS10740 (2231165)	2231165..2231527	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KU529_RS10745 (2231546)	2231546..2232145	+	600	WP_000162810.1	DsbA family protein	-
KU529_RS10750 (2232173)	2232173..2232253	+	81	WP_223197974.1	hypothetical protein	-
KU529_RS10755 (2232418)	2232418..2232669	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU529_RS10760 (2232669)	2232669..2232935	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
KU529_RS10765 (2232970)	2232970..2233041	+	72	Protein_2117	hypothetical protein	-
KU529_RS10770 (2233230)	2233230..2234777	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU529_RS10775 (2234963)	2234963..2235430	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
KU529_RS10780 (2235514)	2235514..2235696	+	183	WP_000230292.1	hypothetical protein	-
KU529_RS10785 (2235913)	2235913..2236737	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
KU529_RS10790 (2236971)	2236971..2237507	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T208155 WP_000113265.1 NZ_CP077856:2232669-2232935 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208155 NZ_CP103582:4027239-4027457 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208155 WP_000584499.1 NZ_CP077856:2232418-2232669 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208155 NZ_CP103582:4026838-4027212 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 7BWF

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60