Detailed information of TA system TA208147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2756949..2757466
• Replicon: chromosome
• Accession: NZ_CP077855
• Organism: Staphylococcus aureus strain L6

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: KU520_RS13695
• Protein ID: WP_000113265.1
• Coordinates: 2757200..2757466 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KU520_RS13690
• Protein ID: WP_000584499.1
• Coordinates: 2756949..2757200 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU520_RS13660 (2752647)	2752647..2753366	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KU520_RS13665 (2753363)	2753363..2754094	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KU520_RS13670 (2754191)	2754191..2755441	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
KU520_RS13675 (2755696)	2755696..2756058	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KU520_RS13680 (2756077)	2756077..2756676	+	600	WP_000162810.1	DsbA family protein	-
KU520_RS13685 (2756704)	2756704..2756784	+	81	WP_223197974.1	hypothetical protein	-
KU520_RS13690 (2756949)	2756949..2757200	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KU520_RS13695 (2757200)	2757200..2757466	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
KU520_RS13700 (2757501)	2757501..2757572	+	72	Protein_2660	hypothetical protein	-
KU520_RS13705 (2757761)	2757761..2759308	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KU520_RS13710 (2759494)	2759494..2759961	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
KU520_RS13715 (2760045)	2760045..2760227	+	183	WP_000230292.1	hypothetical protein	-
KU520_RS13720 (2760444)	2760444..2761268	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
KU520_RS13725 (2761502)	2761502..2762038	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T208147 WP_000113265.1 NZ_CP077855:2757200-2757466 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T208147 NZ_CP103582:326904-327272 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT208147 WP_000584499.1 NZ_CP077855:2756949-2757200 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT208147 NZ_CP103582:326686-326907 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60