Detailed information of TA system TA208057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 26912..27570
• Replicon: plasmid pDETAB7b
• Accession: NZ_CP077836
• Organism: Acinetobacter baumannii strain DETAB-P65

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KU217_RS19145
• Protein ID: WP_000312250.1
• Coordinates: 27211..27570 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU217_RS19140
• Protein ID: WP_001096429.1
• Coordinates: 26912..27211 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU217_RS19110 (KU217_19040)	22932..23582	+	651	WP_139845924.1	hypothetical protein	-
KU217_RS19115 (KU217_19045)	23622..24131	+	510	WP_001043199.1	hypothetical protein	-
KU217_RS19120 (KU217_19050)	24212..24766	+	555	WP_038345807.1	hypothetical protein	-
KU217_RS19125 (KU217_19055)	24816..25352	+	537	WP_000731981.1	hypothetical protein	-
KU217_RS19130 (KU217_19060)	25973..26455	+	483	WP_001052677.1	hypothetical protein	-
KU217_RS19135 (KU217_19065)	26548..26811	-	264	WP_000110863.1	hypothetical protein	-
KU217_RS19140 (KU217_19070)	26912..27211	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
KU217_RS19145 (KU217_19075)	27211..27570	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KU217_RS19150 (KU217_19080)	27771..28337	+	567	WP_000710385.1	hypothetical protein	-
KU217_RS19155 (KU217_19085)	28386..28568	+	183	WP_000373385.1	hypothetical protein	-
KU217_RS19160 (KU217_19090)	28635..29333	+	699	WP_000873188.1	hypothetical protein	-
KU217_RS19165 (KU217_19095)	29472..29855	+	384	WP_000654348.1	hypothetical protein	-
KU217_RS19170 (KU217_19100)	29922..30680	+	759	WP_001053128.1	hypothetical protein	-
KU217_RS19175 (KU217_19105)	30732..31067	+	336	WP_000653925.1	hypothetical protein	-
KU217_RS19180 (KU217_19110)	31932..32246	+	315	WP_000708715.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73444	73444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T208057 WP_000312250.1 NZ_CP077836:c27570-27211 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T208057 NZ_CP103560:c5325975-5325592 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT208057 WP_001096429.1 NZ_CP077836:c27211-26912 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT208057 NZ_CP103560:c5326217-5325975 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA