Detailed information of TA system TA208050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2606..3257
• Replicon: plasmid pDETAB9b
• Accession: NZ_CP077831
• Organism: Acinetobacter baumannii strain DETAB-E51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: KU058_RS20770
• Protein ID: WP_000269904.1
• Coordinates: 2606..2962 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU058_RS20775
• Protein ID: WP_001129974.1
• Coordinates: 2955..3257 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU058_RS20755 (KU058_20650)	1..936	+	936	WP_001292329.1	replication initiation protein RepM	-
KU058_RS20760 (KU058_20655)	1014..1532	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
KU058_RS20765 (KU058_20660)	1742..2041	+	300	WP_000358348.1	hypothetical protein	-
KU058_RS20770 (KU058_20665)	2606..2962	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KU058_RS20775 (KU058_20670)	2955..3257	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
KU058_RS20780 (KU058_20675)	3250..3459	+	210	WP_000069474.1	hypothetical protein	-
KU058_RS20785 (KU058_20680)	3785..4246	-	462	WP_000761346.1	DIP1984 family protein	-
KU058_RS20790 (KU058_20685)	4551..6962	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
KU058_RS20795 (KU058_20690)	7090..7404	-	315	WP_001983304.1	BrnA antitoxin family protein	-
KU058_RS20800 (KU058_20695)	7391..7678	-	288	WP_000438827.1	BrnT family toxin	-
KU058_RS20805 (KU058_20700)	7946..8245	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11193	11193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T208050 WP_000269904.1 NZ_CP077831:2606-2962 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T208050 NZ_CP103560:2465080-2465397 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT208050 WP_001129974.1 NZ_CP077831:2955-3257 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT208050 NZ_CP103560:2465473-2465769 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: N8YFZ1