Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1388300..1388897 Replicon chromosome
Accession NZ_CP077824
Organism Klebsiella pneumoniae strain FF1009

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KU661_RS06540 Protein ID WP_004142563.1
Coordinates 1388300..1388617 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KU661_RS06545 Protein ID WP_004142561.1
Coordinates 1388610..1388897 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU661_RS06525 (KU661_06510) 1384000..1385427 - 1428 WP_065800932.1 MFS transporter -
KU661_RS06530 (KU661_06515) 1385536..1386402 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KU661_RS06535 (KU661_06520) 1387072..1388115 + 1044 WP_102008192.1 DUF2157 domain-containing protein -
KU661_RS06540 (KU661_06525) 1388300..1388617 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KU661_RS06545 (KU661_06530) 1388610..1388897 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KU661_RS06550 (KU661_06535) 1389035..1389688 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KU661_RS06555 (KU661_06540) 1389808..1390176 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KU661_RS06560 (KU661_06545) 1390176..1390757 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
KU661_RS06565 (KU661_06550) 1390937..1392052 + 1116 WP_074422059.1 MBL fold metallo-hydrolase -
KU661_RS06570 (KU661_06555) 1392083..1392424 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KU661_RS06575 (KU661_06560) 1392442..1392690 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1379742..1388897 9155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T208031 WP_004142563.1 NZ_CP077824:1388300-1388617 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T208031 NZ_CP103559:c2430615-2430421 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT208031 WP_004142561.1 NZ_CP077824:1388610-1388897 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT208031 NZ_CP103559:2430244-2430422 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References